Comment on: A novel dysferlin-mutant pseudoexon bypassed with antisense oligonucleotides Virginie Kergourlay, Ga€ elle Blandin, V eronique Blanck, Nicolas L evy, Marc Bartoli & Martin Krahn* Aix Marseille Universit e, GMGF, 13385 Marseille, France Inserm, UMR_S 910, 13385 Marseille, France AP-HM, D epartement de G en etique M edicale et de Biologie Cellulaire, Hôpital d’Enfants de la Timone, 13...

Primary effusion lymphoma (PEL) is a distinct clinicopathologic entity associated with Kaposi's sarcoma-associated herpes virus (KSHV). Several cytokines, including interleukin-6 (IL-6), basic fibroblast growth factor (bFGF), and platelet-derived growth factor (PDGF) may be important for survival of KS cells. However, little is known about the interaction of cytokines with KSHV-infected lymphoc...

OBJECTIVE During coagulation, factor IX (FIX) is activated by 2 distinct mechanisms mediated by the active proteases of either FVIIa or FXIa. Both coagulation factors may contribute to thrombosis; FXI, however, plays only a limited role in the arrest of bleeding. Therefore, therapeutic targeting of FXI may produce an antithrombotic effect with relatively low hemostatic risk. APPROACH AND RESU...

Carbohydrate-oligonucleotide conjugates and glycodendrimers were synthesized utilizing a DNA synthesizer. The synthesis of multivalent glycoconjugates on solid-phase allows custom tailoring of their structure to the requirements of biological assays within hours, as opposed to traditional approaches that require weeks or months of work in the laboratory. Therefore it will become much easier to ...

Antisense oligonucleotides complementary to the 5' end of the mRNA encoding the Ly-6A protein were used to block the expression of that protein. Using this approach we could inhibit the expression of Ly-6A by 60-80% in antigen-primed lymph node (LN) T cells as well as in the D10 T cell clone. Inhibition of Ly-6 expression resulted in the inability to restimulate in vitro, antigen-primed T cells...

Chemically modified antisense oligonucleotides (ASOs) are widely used as a tool to functionalize microRNAs (miRNAs). Reduction of miRNA level after ASO inhibition is commonly reported to show efficacy. Whether this is the most relevant endpoint for measuring miRNA inhibition has not been adequately addressed in the field although it has important implications for evaluating miRNA targeting stud...

Pyrene-labeled 2'-O-methyloligoribonucleotide (OMUpy) and 5'-Ru(II) complex labeled oligodeoxyribonucleotie (Ru-probe) were prepared as the fluorescence probes to detect acceptor sites of antisense molecules on native folded RNA under the physiological condition. OMUpy showed the remarkable increase of the fluorescence intensity (334-fold at 375 nm) only when hybridized with complementary oligo...

Reply to comment on: A novel dysferlin mutant pseudoexon bypassed with antisense oligonucleotides Janice A. Dominov, € Ozg€ un Uyan, Peter C. Sapp, Diane McKenna-Yasek, Babi Ramesh Reddy Nallamilli, Madhuri Hegde & Robert H. Brown Jr Neurology Department, University of Massachusetts Medical School, Worcester, Massachusetts Department of Human Genetics, Emory University School of Medicine, Atlan...