Proposing that a blend of the chemical diversity of small synthetic molecules with the immunological characteristics of the antibody molecule will lead to therapeutic agents with superior properties, we here present a device that equips small synthetic molecules with both effector function and long serum half-life of a generic antibody molecule. As a prototype, we developed a targeting device t...

To examine Gojobori and Nei's hypothesis that the immunoglobulin heavy-chain variable-region (VH) genes in mammals are subject to diversity-enhancing selection, we studied the rates of synonymous and nonsynonymous nucleotide substitution in the complementarity-determining regions (CDRs) and in the framework regions (FRs) of mouse and human VH genes. The results obtained indicate that the non-sy...

Expression of toxic gene products affects bacterial cell growth and phage display, causing a strong selection against plasmid maintenance and integrity. During phage propagation steps, in particular, phagemid instability can dramatically affect diversity of antibody libraries or even lead to the deletion of antibody genes. We constructed a modified phage display vector by introducing a strong t...