Rhesus (Rh) haemolytic disease has been greatly reduced in incidence by the prophylactic programme with anti-D immunoglobulin, but it will not be eliminated. Even with careful documentation and close liaison between medical, nursing and laboratory personnel, there are occasional failures to administer anti-D to Rh-negative women at risk of sensitization following either delivery (Crowle, 1974) ...

Toxoplasma gondii is an apicomplexan parasite that infects human and almost all warm-blooded animals. The life cycle of the parasite includes an asexual reproduction in intermediate hosts (Mammals and birds) and a sexual reproduction in definitive hosts (Felidae). Cats are both the intermediate and the definitive host for T. gondii. The aim of this study was to investigate anti-T. gondii antibo...

BACKGROUND The phenomena of co-incidence of transfusion-induced allo- and autoantibodies, blockage and/or loss of red blood cell (RBC) antigens are conspicuous and may result in confusion and misdiagnosis. CASE REPORT A 67-year-old female was transferred to the intensive care unit due to hemolysis which developed 2 days following transfusion of three Rh(D)-negative RBC units in the presence o...

Out of 177 Rh(D)-negative patients studied, 96 were successfully followed-up after spontaneous or therapeutic abortion. Rh antibodies were detected by the indirect Coombs test in two patients and by an enzyme technique only in a further seven, an overall incidence of 9.4%.The prophylactic use of anti-D immunoglobulin is now recommended for Rh-negative non-immunized patients undergoing abortion,...

 This study was aimed to detect anti-Toxoplasma gondii antibodies in cats infected with Rh strain of T. gondii by using an immunoblotting method. For this, cats were experimentally infected using tachyzoites harvested from Vero cell cultures. Tachyzoites were then lysed and transferred to polyacrylamide gels followed by blotting to PVDF membranes. An immunoblotting was performed using these mem...

The purpose of pretransfusion compatibility testing is to prevent immune mediated hemolytic transfusion reactions. Our study aimed to evaluate the gel test for detection of clinically significant antibodies in repeatedly transfused patients. We investigated 200 thalassemic patients in whom, blood group, Rh-D, Rh phenotype determination, antibody screening and identification were done using an I...

Description of Procedure or Service Rhesus (Rh) D-negative women who are exposed to RhD-positive red blood cells can develop anti-Rh antibodies, which can cross the placenta and cause fetal anemia. If undiagnosed and untreated, alloimmunization can cause significant perinatal morbidity and mortality. Determining the Rh status of the fetus may guide subsequent management of the pregnancy. The us...

The red cells of 63 members of 11 families were tested with (125)I-labeled anti-Rh(0)(D). Families with a history of hemolytic disease of the newborn due to fetomaternal Rh incompatibility were selected for study. In such families it was possible to determine the antibody binding to the Rh(0)(D) heterozygous red cells of the children and to compare within each family this value with the antibod...