نتایج جستجو برای: annexin vpropidium iodide

تعداد نتایج: 20402  

Journal: :Human reproduction 2001
L Ramos A M Wetzels

BACKGROUND In this study we present the physiological changes observed in ejaculated spermatozoa of normospermic men after exposure to hydrogen peroxide (H(2)O(2)) or gamma irradiation. METHODS Motility changes as well as membrane and DNA-damage were determined in spermatozoa after incubation with 25 micromol/l of H(2)O(2) during increasing intervals of time (0--60 min and after 24 h) or afte...

2015
TAMILSELVAN SUBRAMANI SWEE KEONG YEAP WAN YANG HO CHAI LING HO CHE PUTEH OSMAN NOR HADIANI ISMAIL NIK MOHD AFIZAN NIK ABDUL RAHMAN NOORJAHAN BANU ALITHEEN

Tamoxifen (TAM) is the mainline drug treatment for breast cancer, despite its side effects and the development of resistance. As an alternative approach, in the present study a novel combination therapy was established through combining TAM with nordamnacanthal (NDAM) in order to investigate the additive effect of these drugs in MCF-7 human breast cancer cells. A significant dose-dependent redu...

2006
Roopa Subbegowda Thomas O. Frommel

Chemoprevention of colorectal cancer using aspirin has been demon strated in rodents and has been suggested by data from epidemiolÃ3gica! studies. The mechanism that accounts for this prevention is unknown, but it is thought to relate to an irreversible inhibition of cyclooxygenase and, subsequently, prostaglandin production. The effect of aspirin on the growth of human colonie tumor cells was ...

Journal: :International journal of clinical and experimental pathology 2015
Qiong-Yu Lan Que-Ling Liu Jing Cai An-Wen Liu

Coumarins induce apoptosis by activating mitochondrial pathway and caspase-3-dependent apoptotic pathway. In the present study, we first time investigated the effect of 3-cinnamoyl-4-hydroxy-6-methyl-2H-pyran-2-one (CHP) on induction of apoptosis in human ovarian carcinoma cells. The data from MTT assay revealed a significant inhibitory effect on cell viability at 30 (87%) and 50 μM (74%) conce...

2011
Michele Cea Debora Soncini Floriana Fruscione Lizzia Raffaghello Anna Garuti Laura Emionite Eva Moran Mirko Magnone Gabriele Zoppoli Daniele Reverberi Irene Caffa Annalisa Salis Antonia Cagnetta Micaela Bergamaschi Salvatore Casciaro Ivana Pierri Gianluca Damonte Filippo Ansaldi Marco Gobbi Vito Pistoia Alberto Ballestrero Franco Patrone Santina Bruzzone Alessio Nencioni

Aberrant histone deacetylase (HDAC) activity is frequent in human leukemias. However, while classical, NAD(+)-independent HDACs are an established therapeutic target, the relevance of NAD(+)-dependent HDACs (sirtuins) in leukemia treatment remains unclear. Here, we assessed the antileukemic activity of sirtuin inhibitors and of the NAD(+)-lowering drug FK866, alone and in combination with tradi...

Journal: :Journal of lipid research 1999
J Alcouffe S Caspar-Bauguil V Garcia R Salvayre M Thomsen H Benoist

Oxidized low density lipoproteins (oxLDLs) and activated T lymphocytes are present in early atherosclerotic plaques. It has been shown that oxLDLs are cytotoxic to cultured vascular cells but their possible toxic action on T lymphocytes has not been described. Peripheral blood lymphocytes from healthy individuals were stimulated in vitro with the polyclonal activator phytohemagglutinin and trea...

Journal: :Human reproduction 2006
Masao Izawa Tasuku Harada Imari Deura Fuminori Taniguchi Tomio Iwabe Naoki Terakawa

BACKGROUND The survival of endometriotic cells in the ectopic site has been investigated from the aspect of susceptibility of endometriotic tissues to apoptosis. In order to investigate the nature of abnormal survival of endometriotic cells in ectopic locations, we compared drug-induced apoptosis in endometrial and endometriotic cells. METHODS Endometrial stromal cells were obtained from norm...

2010
O Ciepiela A Zawadzka-Krajewska I Kotula M Wasik U Demkow

BACKGROUND The resistance of T lymphocytes to Fas-mediated apoptosis is an important feature of atopic asthma. The only effective causative treatment of atopic diseases is immunotherapy. Clinical efficacy of sublingual immunotherapy (SLIT) has been already proven, but there is still limited number of studies on its influence on lymphocytes function. OBJECTIVES The aim of the study was to eval...

Journal: :Cardiovascular research 2007
Stefan Preiss Dmitry Namgaladze Bernhard Brüne

OBJECTIVE Modification of low density lipoprotein (LDL) by phospholipases confers pro-atherogenic properties, although signalling pathways of phospholipase-modified LDL (PLA-LDL) remain obscure. We questioned whether members of the protein kinase C (PKC) family are involved in PLA-LDL-induced Akt phosphorylation and survival of THP-1 monocytic cells. METHODS Akt phosphorylation in THP-1 cells...

2008
Bong Rim Kim Jae Won Shim Dong Kyung Sung Sung Shin Kim Ga Won Jeon Myo Jing Kim Yun Sil Chang Won Soon Park Eung Sang Choi

PURPOSE This study was undertaken to determine the neuroprotective effect of granulocyte stimulating factor (G-CSF) on neonatal hypoxic-ischemic brain injury. MATERIALS AND METHODS Seven-day-old male newborn rat pups were subjected to 110 minutes of 8% oxygen following a unilateral carotid artery ligation. Apoptosis was identified by performing terminal deoxynucleotidyl transferase-mediated d...

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