Since immobilized metal ion affinity chromatography (IMAC) was first reported, several modifications have been developed. Among them, Ni(2+) immobilized by chelation with nitrilotriacetic acid (NTA) bound to a solid support has become the most common method for the purification of proteins carrying either a C- or N-terminal histidine (His) tag. Despite its broad application in protein purificat...

An important goal is to identify the direct activation domain (AD)-interacting components of the transcriptional machinery within the context of native complexes. Toward this end, we first demonstrate that the multisubunit TFIID, SAGA, mediator, and Swi/Snf coactivator complexes from transcriptionally competent whole-cell yeast extracts were all capable of specifically interacting with the prot...

RATIONALE Bunyaviruses have become a major threat to both humans and livestock in Europe and the Americas. The nucleocapsid (N) protein of these viruses is key to the replication cycle and knowledge of the N oligomerisation state is central to understanding the viral lifecycle and for development of therapeutic strategies. METHODS Bunyamwera virus and Schmallenberg virus N proteins (BUNV-N an...

Rhp14 of Schizosaccharomyces pombe is homologous to human XPA and Saccharomyces cerevisiae Rad14, which act in nucleotide excision repair of DNA damages induced by ultraviolet light and chemical agents. Cells with disrupted rhp14 were highly sensitive to ultraviolet light, and epistasis analysis with swi10 (nucleotide excision repair) and rad2 (Uve1-dependent ultraviolet light damage repair pat...

Leptospirosis is a worldwide zoonosis caused by pathogenic Leptospira. The whole-genome sequence of L. interrogans serovar Copenhageni together with bioinformatics tools represent a great opportunity to search for novel antigen candidates that could be used as subunit vaccine against leptospirosis. We focused on six genes encoding for conserved hypothetical proteins predicted to be exported to ...

Intracellular delivery of functional macromolecules using peptide transduction domains (PTDs) is an exciting technology with both experimental and therapeutic applications. Recent data indicate that PTD-mediated transduction occurs via fluid-phase macropinocytosis involving an intracellular pH drop to approximately 5. Nitrilotriacetic acid (NTA)-coordinated metals avidly bind hexahistidine-tagg...

This article examines Schwedenreiter (2018), the second novel by German-Austrian writer Hanna Sukare (b. 1957). It focuses on eponymous grandson of a Wehrmacht deserter and his quest for grandfather’s story to be rehabilitated in village from which he originated. comes wake rehabilitation deserters Austria at national level 2009, is explicitly thematized narrative. The analysis draws specifical...

Lanka, and emigrate to the UK in 1981. He worked at Manor and St Ebba’s hospitals as a consultant psychiatrist in learning disabilities from 1981 until 1995. Satkunam was born in May 1926 and educated in Jaffna, Northern Sri Lanka, and qualified in medicine in Colombo. He obtained his MRCPsych in 1963, completing postgraduate study at the Institute of Psychiatry, University of London, and retur...

President Light, colleagues, and distinguished international guests: It is a great and humbling honor to address you and to serve as President of the American Orthopaedic Association (AOA). Jim Beaty, President of The American Academy of Orthopaedic Surgeons, used an aphorism in his recent address to the membership that I would like to share with you: ‘‘If you see a turtle on a fence post, it d...