In the present study purification and characterization of a novel extracellular pullulanase enzyme from Bacillus thuringiensis was carried out for use in detergents. Maximum production (5.71 U/ml) obtained medium containing tryptone as carbon energy source having pH 6.0 when it inoculated with 3% overnight grown inoculum incubated at 37°C 24 hrs.  Optimal conditions pullalanse activity wer...

The Chilean sclerophyllous matorral is a Mediterranean semiarid ecosystem affected by erosion, with low soil fertility, and limited by nitrogen. However, limitation of resources is even more severe for desert soils such as from the Atacama Desert, one of the most extreme arid deserts on Earth. Topsoil organic matter, nitrogen and moisture content were significantly higher in the semiarid soil c...

α-Amylases are widely used enzymes in starch saccharification and baking industries, and in textile desizing. These are endoenzymes which cleave α-1,4-glycosidic linkages randomly in starch, thus, generate maltose and malto oligosaccharides. The commercially available α-amylases have certain limitations like a limited activity at low pH and Ca2+dependence, therefore, the search for novel acid s...

Among the microbial enzymes protease and amylase are most valuable which have been has diversified applications used extensively because of their capabilities in degradation organic wastes, application biofuels, agricultural, pharmaceuticals, chemical biotechnological industries. The aim current research work was purification, characterization alkaline proteases extracted from Bacillus cereus A...

In the present study 10 bacterial isolates were isolated from the three different-different places soil sample by serial dilution and agar plate technique. These bacterial isolated colony were identified on the basis of colony morphology given by Bergey’smanual method and named as MJKS01 to MJKS10. Screening of the colony for the cellulolytic activity was done on the MAM (minimal agar medium) s...

early in this study, an α-amylase from bacillus megaterium who (bmw) was isolated from hot springs of ramsar (north of iran), and its gene was cloned in e.coli. based on its conserved sequence regions and substrate specificity, it was classified as intermediary group enzymes with the specificity of oligo-1,6-glucosidase and neopullulanase subfamilies. in the sixth conserved region (83-qvngiwmmp...

The gene for beta-amylase was isolated from Bacillus polymyxa by molecular cloning in B. subtilis. B. subtilis cells containing this gene express and secrete an amylase which resembles the B. polymyxa beta-amylase and barley beta-amylase in terms of the products it generates during carbohydrate hydrolysis. Starch hydrolysis with this beta-amylase produces maltose, not glucose, whereas maltotrio...

An integrable plasmid, pOK4, which replicated independently in Escherichia coli was constructed for generating transcriptional fusions in vivo in Bacillus DNA. It did not replicate independently in Bacillus subtilis, but it could be made to integrate into the chromosome of B. subtilis if sequences homologous to chromosomal sequences were inserted into it. It had a selectable marker for chloramp...

BACKGROUND Lectins are carbohydrate binding proteins or glycoproteins that bind reversibly to specific carbohydrates present on the apposing cells, which are responsible for their ability to agglutinate red blood cells, lymphocytes, fibroblasts, etc. Interest in lectins has been intensified due to their carbohydrate specificity as they can be valuable reagents for the investigation of cell surf...