The toxicity of extracellular spermine, determined in the presence of fetal calf serum, was studied using three cell lines: FM3A, L1210, and NIH3T3 cells. Amine oxidase in fetal calf serum produces aminodialdehyde generating acrolein spontaneously, H(2)O(2), and ammonia from spermine. Spermine toxicity was prevented by aldehyde dehydrogenase, but not by catalase. Similar concentrations of sperm...

Ammonia-lyase activity for L-phenylalanine, m-hydroxyphenylalanine and L-tyrosine was demonstrated in cell-free extracts ofSporobolomyces roseus. Cultures of this organism converted DL-[ring-14C]phenylalanine and L-[U-14C]tyrosine into the corresponding cinnamic acid. Tracer studies showed that these compounds were further metabolized to [14C]protocatechuic acid. Benzoic acid andp-hydroxybenzoi...

Tert-butyl carbazate (1) reacted with ammonium thiocyanate and phenyl isothiocyanate to afford the corresponding 5-tert-butoxy-4H-1, 2, 4-triazole-3-thiol (3) 5-tert-butoxy-4-phenyl-2H1, 2, 4-triazole-3(4H)-thione 5 respectively. Compound (1) also reacted with carbon disulfide and potassium hydroxide to afford 5-tert-butoxy-1, 3, 4-oxadiazole-2(3H)-thione 6. Treatment of 6 with methyl iodide, 2...

The product formed by incubation of dolichol monophosphate glucose with liver microsomes was studied. It is insoluble in most solvents, but is soluble in a chloroform-methanol mixture with a high content of water. Treatment with ammonia gave rise to the formation of a water soluble, negatively charged compound of molecular weight 3550. The negative charge could be removed by treatment with phos...

1. The products of the lactoperoxidase-catalysed oxidation of thiocyanate by hydrogen peroxide were sulphate, carbon dioxide and ammonia. Cyanate, sulphite and a compound showing increased extinction at 235m,u (the '235 compound') were intermediate oxidation products. 2. Two of the intermediates acted as electron acceptors in the oxidation of NADH2. Thus NADH2 was oxidized by sulphite in the pr...

1. The products of the lactoperoxidase-catalysed oxidation of thiocyanate by hydrogen peroxide were sulphate, carbon dioxide and ammonia. Cyanate, sulphite and a compound showing increased extinction at 235mmu (the ;235 compound') were intermediate oxidation products. 2. Two of the intermediates acted as electron acceptors in the oxidation of NADH(2). Thus NADH(2) was oxidized by sulphite in th...

Among the various physiological-biochemical changes associated with the invasion of land from an aquatic environment is that dealing with the nature of the nitrogen excretion product of protein and amino acid catabolism. In general terms (l-4), the excretion of ammonia (ammonotelism), urea (ureotelism), or uric acid (uricotelism) is related to the availability of water to an organism. Ammonia c...

Among the various physiological-biochemical changes associated with the invasion of land from an aquatic environment is that dealing with the nature of the nitrogen excretion product of protein and amino acid catabolism. In general terms (l-4), the excretion of ammonia (ammonotelism), urea (ureotelism), or uric acid (uricotelism) is related to the availability of water to an organism. Ammonia c...

Utilization of cyanide as a nitrogen source by Pseudomonas fluorescens NCIMB 11764 occurs via oxidative conversion to carbon dioxide and ammonia, with the latter compound satisfying the nitrogen requirement. Substrate attack is initiated by cyanide oxygenase (CNO), which has been shown previously to have properties of a pterin-dependent hydroxylase. CNO was purified 71-fold and catalyzed the qu...

Nitrogen (N) is a nutrient element necessary for plant growth and development. However, excessive inputs of N will lead to inefficient use and large N losses to the environment, which can adversely affect air and water quality, biodiversity and human health. To examine the effects of controlled-release fertilisers (CRF) on yield, we measured ammonia volatilisation, N use efficiency (NUE) and ph...