A previously constructed mutation of the histone H4 gene has been the subject of a detailed analysis. The mutation involves an H4 gene encoding two amino acid changes which substitute aspartic acid for glycine at positions 13 and 42 of the polypeptide. These substitutions result in a nonfunctional H4 protein when assayed in Saccharomyces cerevisiae. In contrast, an analysis of an amino terminal...

Here, we explored the genomic loci and genes responsible for differences in Cs accumulation seeds of two soybean (Glycine max (L.) Merr.) cultivars, namely ‘Enrei’ (high accumulator) ‘Onihadaka’ (low accumulator), using quantitative trait (QTL)-seq. For analysis, F3 F5 generations were generated by crossing cultivars followed self-fertilization. In putative QTL regions generation, Glyma.11G0991...

The substitution of each constituent amino acid residue of gramicidin S (GS), cyclo(-Val(1,1')-Orn(2,2')-Leu(3,3')-D-Phe(4,4')-Pro(5,5')-)(2) with Lys residue indicated that each side chain structure of the constituent amino acid residues affect largely the antibiotic activity and hemolytic activity of GS. Further, the substitution of D-Phe(4,4') and Pro(5,5') residues with basic amino acid res...

Grapevine powdery mildew (GPM), caused by the fungus Erysiphe necator, is a constant threat to worldwide production of grape berries, requiring repeated use fungicides for management. The frequent fungicide applications have resulted in resistance commonly used quinone outside inhibitor (QoI) and associated with single-nucleotide polymorphisms (SNPs) mitochondrial cytochrome b gene (cytb). In t...

Utilization of fecundity genes such as GDF9 and BMP15 can help improve reproductive traits in sheep breeding programme. To evaluate effects of missense mutations on protein function, the polymorphisms of GDF9 and BMP15 genes were screened in twelve mehraban sheep using DNA sequencing, followed by protein structure modeling. Six single nucleotide polymorphism (SNPs) known as FecG mutations (G1-G...