the response of five inbred sunflower seedling lines, including ac 4122, c, ha 89, ha 410, ha 411, to inoculation with sclerotinia sclerotiorum culture filtrate containing endogenous oxalic acid was compared with the exogenous application of synthetic oxalic acid. the reaction of seedlings was evaluated in terms of dry and fresh plant weights and the total chlorophyll concentration relative to ...

BACKGROUND Deaths from inherited metabolic disorders may remain undiagnosed after postmortem examination and may be classified as sudden infant death syndrome. Tandem mass spectrometry (MS/MS) may reveal disorders of fatty acid oxidation in deaths of previously unknown cause. METHODS We obtained filter-paper blood from 7058 infants from United States and Canadian Medical Examiners. Acylcarnit...

The isolation and sequence of a cDNA clone encoding the complete glyoxysomal malate dehydrogenase [gMDH; (S)-malate:NAD+ oxidoreductase, EC 1.1.1.37] of watermelon cotyledons are presented. Partial cDNA clones were synthesized in a three part strategy, taking advantage of the polymerase chain reaction technology with oligonucleotides based on directly determined amino acid sequences. Subsequent...

The compartmentation of key processes in sugar, organic acid and amino acid metabolism was studied during the development of the flesh and seeds of grape (Vitis vinifera L.) berries. Antibodies specific for enzymes involved in sugar (cell wall and vacuolar invertases, pyrophosphate: fructose 6-phosphate phosphotransferase, aldolase, NADP-glyceraldehyde-P dehydrogenase, cytosolic fructose 1,6-bi...

The enzymes that generate retinoic acid during development have been identified as members of the aldehyde dehydrogenase (ALDH) family. The developmental expression patterns of two ALDHs that function as retinaldehyde dehydrogenases, RALDH1 and RALDH2, have been described. Here we report the cloning and expression of a third retinaldehyde dehydrogenase from the mouse called RALDH3 that shares 9...

Abstract ?-Alanine (3-aminopropionic acid) is the only naturally occurring ?-amino acid and an important precursor for synthesis of a variety nitrogen-containing chemicals. Fermentative production ?-alanine from renewable feedstocks such as glucose has attracted significant interest in recent years. Methanol become emerging promising feedstock biomanufacturing alternative to glucose. In this wo...

study was undertaken to establish the optimum dietary indispensable amino acid (IAA) profile for yellow fin seabream broodstock based on the amino acid (AA) profile of broodstock, eggs and larvae of Acanthopagrus latus. Three isonitrogenous (40%) and isolipid (20%), named diets 1, 2 and 3 were formulated with different ratios of total essential amino acids per total non-essential amino acid...

Two maize cDNAs were isolated and sequenced that had open reading frames with approximately 37% amino acid identity to mammalian pyruvate dehydrogenase kinases. Both maize kinase sequences contain the five domains with conserved signature residues typical of procaryotic two-component histidine kinases. Sequence comparisons identified six other highly conserved motifs that are proposed to be spe...

Rats were given radioactive r.-leucine by intravenous injection. At times ranging from 30 set to 4 hours after injection, livers were frozen in situ, lyophilized, and separated into nuclear and cytoplasmic fractions in nonaqueous solvents. In each of the fractions thus prepared, the following were determined: (a) concentration of free labeled amino acid, (b) specific activity of the total prote...