Structure activity relationships for semicarbazide-sensitive amine oxidase/vascular adhesion protein-1 (SSAO/VAP-1) were studied using a library of arylalkylamine substrates, with the aim of contributing to the discovery of more efficient SSAO substrates. Experimental data were contrasted with computational docking studies, thereby allowing us to examine the mechanism and substrate-binding affi...

Aromatic amine acetylation has been recognized for many years as an important metabolic polymorphism in humans because of its relationship to disease. This system serves as a model in risk assessment because of its role in drug and carcinogen activation and detoxification and because of the case with which it is measured. However, possible interactions of NAT1-NAT2 phenotypes or genotypes illus...

In atomic force microscopy (AFM) a sharp cantilever tip is used to scan surfaces at the atomic level. One further application is force spectroscopy, in which force-distance curves between binding partners located on the cantilever and substrate surface are determined. This requires specifically immobilized molecules. Herein we describe the covalent binding of single adenosine and thymidine nucl...

Membrane-spanning phospholipid bolaamphiphiles either alone or as a constituent of a multicomponent lipid membrane may prove to be facile building blocks for generating robust bioactive membrane-mimetic assemblies. We have previously reported the synthesis of asymmetric dialkyl phospholipid bolaamphiphiles that contain ester linked phosphatidylcholine and amine functionalities at opposite chain...

The carbocyclic analogues of phosphoribosylamine, glycinamide ribonucleotide, and formylglycinamide ribonucleotide have been prepared as the racemates. Carbocyclic phosphoribosylamine was utilized as a substrate by the monofunctional glycinamide ribonucleotide synthetase from Escherichia coli as well as the glycinamide ribonucleotide synthetase activity of the eucaryotic trifunctional enzyme of...

Ureido-functionalized compounds play an indispensable role in important biochemical processes, as well chemical synthesis and production. Isocyanates, KOCN particular, are the preferred reagents for ureido functionalization of amine-bearing compounds. In this study, we evaluate potential urea a reagent to graft groups onto amines at relatively low temperatures (<100 °C) aqueous media. Urea i...

The organization of nano-objects on macroscopic surfaces is a key challenge for the technological improvement and implementation nanotechnologies. For achieving operational functions, it required to assemble as controllable building blocks in highly ordered superstructures. Herein, we demonstrate growth self-organization metallic nanowires into hexagonal superlattices with tunable characteristi...

Rhbg is a nonerythroid membrane glycoprotein belonging to the Rh antigen family. In the kidney, Rhbg is expressed at the basolateral membrane of intercalated cells of the distal nephron and is involved in NH4+ transport. We investigated the substrate specificity of Rhbg by comparing transport of NH3/NH4+ with that of methyl amine (hydrochloride) (MA/MA+), often used to replace NH3/NH4+, in oocy...

In their studies of the oxidation of adrenalin and other amines by amine oxidase preparations, Blaschko, Richter, and Schlossmann (1) found that l-ephedrine, triethylamine, triisoamylamine, and dl-1-hydroxy-2-hydrindeneamine are not only not oxidized, but inhibit the action of the enzyme upon isoamylamine. The conclusion was drawn that this inhibition was of the competitive type, because some d...