Prion diseases such as bovine spongiform encephalopathy, chronic wasting disease, and scrapie pose serious risks to human and animal health due to a host of disease-specific factors, including the resistance of infectious prions (PrP(Sc)) to natural degradation and to most commercial inactivation procedures. In an attempt to address this concern, a mouse model was used to compare the efficacy o...

This work focuses on the selectivity modification of ceramic membranes after a mild alkaline treatment. Filtration of pure salt-water solutions was carried out with commercial titania membranes before and after the treatment. After treatment, the rejection of NaF significantly decreased, while the rejection of NaCl and NaBr increased. Additionally, NaI and Na₂SO₄ remained close to zero. Pore si...

We compared an alkaline phosphatase (EC 3.1.3.1) isoenzyme from a plasma sample of a patient with carcinoma of the lung, who was suspected of having the Regan-type isoenzyme, with placental serum alkaline phosphatase and with tissue preparations from placenta, liver, bone, and intestine. The placental and Regan phosphatases responded similarly to heat treatment and modification by various inhib...

Earlier studies in this laboratory (1) and by other workers (Z-4) have suggested that the effect of alkaline pH and urea on myosin resulted in major structural alterations in the molecule. Many of these studies were concerned with the attempt at the isolation of subunits or small polypeptide fragments by the treatment of myosin with high urea concentrations for long periods of time or by exposu...

uridine (lUdR) there was an increase in alkaline phosphatase activity after about 24 hr, reaching a maximum usually at about 72 hi. At this concentration, during this time, there was a relatively small inhibition of cell replication. The increment in alkaline phosphatase activity was in large measure prevented by concomitant addition of 10 /¿Mthymidine. Further, lUdR did not increase alkaline ...

Monosodium glutamate (MSG) was administrated chronically for 60 days to Wistar rats and 24 h rhythms of glucose, cholesterol, total protein and alkaline phosphatase were studied. MSG treatment was found to cause acrophase delays in the glucose and alkaline phosphatase rhythms and advances in acrophases of cholesterol and total protein levels. Amplitude and mesor values of these rhythms were fou...

Three monoclonal antibodies to neurofilaments (RT97, BF10 and 147), two of which also recognised neurofibrillary tangles (RT97 and BF10), have all been shown to be specific for phosphorylated epitopes. Treatment of histological sections with alkaline phosphatase prior to immunostaining resulted in reduction of axonal neurofilament staining with all three whilst the neurofibrillary tangles stain...

The study concerns the optical and structural properties of layers obtained by a new efficient surface treatment totally free of chromium species. The process is made up of an anodic oxidation of copper in an alkaline solution followed by an alkaline potassium permanganate dipping post-treatment. Coatings, obtained at the lab and pilot scales, are stable up to 220 C in air and vacuum, present l...

As illustrated in the preceding paper (Firestone, G. L., and Heath, E. C. (1981) J. Biol. Chem. 256, 13961403), the de novo synthesis of a cell surface-localized glycoprotein that exhibits alkaline phosphatase activity was induced by treatment of mouse L-cell cultures with dibutyryl cyclic AMP. When the exposure of L-cell cultures to dibutyryl cyclic AMP was carried out in the presence of inhib...

The advantages and drawbacks of existing wet chemical phosphorus (P) recovery technologies, their applicability to different types of sewage sludge ash (SSA) and the role of the decay products of detergent zeolites as a source of reactive Al in SSA are analyzed. Since neither a purely acidic nor a purely alkaline treatment are able to provide satisfactory technical solutions a wet chemical phos...