This paper reports the isolation and characterization of a new o-nitrobenzaldehyde (ONBA)-degrading bacterium, Alcaligenes sp. ND1. ND1 degraded almost all ONBA (100 mg L(-1)) in M9 medium within 36 hours. The key enzyme(s) involved in the initial biodegradation was a constitutively intracellular enzyme(s). This bacterium has great potential utility for bioremediation.

BACKGROUND In clinical settings, bacterial infections are usually diagnosed by isolation of colonies after laboratory cultivation followed by species identification with biochemical tests. However, biochemical tests result in misidentification due to similar phenotypes of closely related species. In such cases, 16S rDNA sequence analysis is useful. Herein, we report the first case of an Achromo...

The full-length gene encoding the histone deacetylase (HDAC)-like amidohydrolase (HDAH) from Bordetella or Alcaligenes (Bordetella/Alcaligenes) strain FB188 (DSM 11172) was cloned using degenerate primer PCR combined with inverse-PCR techniques and ultimately expressed in Escherichia coli. The expressed enzyme was biochemically characterized and found to be similar to the native enzyme for all ...

5. Soriano, F., J. M. Aguado, C. Ponte, R. Fernandez-Roblas, and J. L. Rodriguez-Tudela. 1990. Urinary tract infection caused by Corynebacterium group D2: report of 82 cases and review. Rev. Infect. Dis. 12:1019-1034. 6. Soriano, F., C. Ponte, P. Ruiz, and J. Zapardiel. 1993. Nonurinary tract infections caused by multiply antibiotic-resistant Corynebacterium urealyticum. Clin. Infect. Dis. 17:8...

Achromobacter xylosoxidans is frequently isolated from the respiratory secretions of cystic fibrosis (CF) patients, but identification with biochemical tests is unreliable. We describe fluorescence in situ hybridization assays for the rapid identification of Achromobacter xylosoxidans and Alcaligenes faecalis. Both assays showed high sensitivities and high specificities with a collection of 155...

The membrane-bound hydrogenase of Alcaligenes eutrophus was solubilized from washed membranes of autotrophically grown cells. The enzyme consists of two types of subunits and is an iron-sulfur protein. A flavin compound was not detected. The enzyme reacts only with few artificial electron acceptors.

To determine the incidence of bacteremia in healthy adults, blood cultures were obtained from 240 patients who had no demonstrable foci of infection. Five patients (2.1%) had positive blood cultures. Staphylococcus epidermidis was isolated from four patients and Alcaligenes faecalis from one. In each of these patients, the organism isolated probably represented contamination rather than bactere...

We report here the isolation and analysis of novel repA host range mutants of pPS10, a plasmid originally found in Pseudomonas savastanoi. Upon hydroxylamine treatment, five plasmid mutants were selected for their establishment in Escherichia coli at 37 degrees C, a temperature at which the wild-type form cannot be established. The mutations were located in different functional regions of the p...

A wastewater from a chemical company (abbreviated as WC) containing approximately 5000 mg NH4-N/L ammonium and a slight amount of BOD was treated with Alcaligenes faecalis No.4 (No.4),which is capable of heterotrophic nitrification and aerobic denitrification. The repeatedbatch operation was conducted at 30°C for 900 h at controlled dissolved oxygen concentration (DO) of 2-3 mg/L in the presenc...

A bacterial strain (designated as Alcaligenes sp. MS-103) isolated from oil sample of the Aghajari oilfield in the south of Iran, was able to produce an effective extracellular lipopolysaccharide biosurfactant (1.2±0.05 g/l) on molasses as a sole carbon source. The highest surface tension reduction to level 20 mN/m was achieved by biosurfactant produced by cells grown on molasses under optimum ...