High hemagglutinin titres against chicken erythrocytes were detected in the sera of Trypanosoma congolense-infected sheep. Adsorption of sheep sera with solubilized T. congolense resulted in marked reduction of hemagglutinin titre. Heat inactivation of the sera at 56 degrees C for 30 min had no demonstrable effects on the hemagglutinin titre. Sera collected from the sheep before trypanosome inf...

A new flexible type of pilus was purified from Vibrio cholerae non-O1, non-0139 strain NAGV14 and characterized. The molecular mass of the pilin was estimated to be 20 kDa, and the antigenicity differed from that of known pili such as toxin-coregulated pili, mannose-sensitive hemagglutinating pili, V10 pili, and Al-1841 pili. The NAGV14 pilus was regarded as a colonization factor because the pu...

A fish pathogen, Vibrio cholerae non-O1, was isolated from diseased ayu fish (Plecoglossus altivelis) collected from rivers in eight prefectural districts of Japan. This organism was found to have biochemical characteristics similar to those of V. cholerae non-O1, except that our isolates were negative for ornithine decarboxylase. Antiserum against an ayu isolate did not agglutinate with the ma...

Purified axial filaments from eight serotypes of Treponema hyodysenteriae and two nonpathogenic intestinal spirochaetes were characterized by SDS-PAGE and Western blotting. Axial filaments of all ten strains had similar SDS-PAGE profiles; five major axial filament polypeptides were identified, with molecular masses of 43.8, 38, 34.8, 32.8 and 29.4 kDa. Hyperimmune gnotobiotic pig serum raised a...

A natural lectin from the seed of peanut Arachis hypogaea was purified by singlestep affinity chromatography using galactoside-coupled agarose. The native molecular mass of purified A. hypogaea lectin (PN-L) was 29 kDa. The lectin PN-L was detected for agglutinating activity, glycoinhibiting action and thermostability. The influence of pH on those activities was also tested. The results showed ...

Lectins are carbohydrate-interacting proteins that play a pivotal role in multiple physiological and developmental aspects of all organisms. They can specifically interact with different bacterial and viral pathogens through carbohydrate-recognition domains (CRD). In addition, lectins are also of biotechnological interest because of their potential use as biosensors for capturing and identifyin...

Naturally occurring agglutinin was analyzed in the hemolymph of crab Varuna litterata by hemagglutination assay (HA). Hemolymph of V. litterata had the capacity to agglutinate a variety of mammalian erythrocytes, rat > rabbit = guinea pig = horse = buffalo = human A = human B > human O > dog > donkey = goat. Among the various tissues tested for HA, maximum HA titer was observed in the hemolymph...

The function of human purified colostral and gastrointestinal IgA has been studied by its ability to agglutinate common gastrointestinal organisms. Agglutinating activity was unaffected by trypsin or acid but it was abolished rapidly by pepsin. Both colostral and gastrointestinal IgA agglutinated a wide range of enteric organisms. Variations in this activity occurred between different individua...

Pilus of Vibrio parahaemolyticus O3:K6 strain LVP9 belonging to the newly identified clone was purified and characterized. The molecular mass of the pilin was estimated to be about 18 kDa by SDS-PAGE, and the isoelectric point of the pilin was 5.0 +/- 0.2. The LVP9 pili were antigenically different from the other V. parahaemolyticus Na2 pili and Ha7 pili as previously reported, nevertheless all...

1. The agglutination of human erythrocytes and the precipitation of the blood group A substance by Type XIV antipneumococcus horse serum are properties of the specific anticarbohydrate immune bodies in the serum. 2. Absorption of Type XIV antipneumococcus horse serum with the homologous bacterial polysaccharide removes the agglutinins for human erythrocytes as well as the precipitins for the gr...