Two procedures were developed for removing DNA from agarose after electrophoretic separation of DNA fragments according to size. Both involve dissolving the DNA-containing agarose in NaI. The preparative technique uses binding of DNA to glass in the presence of NaI. The method is rapid and convenient, and DNA of all molecular weight ranges can be recovered in high yield and without degradation....

A mixed mode dispersive solid phase extraction was introduced for the of multi – class pesticides in mango samples. In following, extracted analytes were more concentrated by liquid microextraction. By use advantages both sample preparation methods, proposed analytical method sensitive with high enrichment factors and less matrix effect. Some biopolymers including chitosan, agarose, cellulose e...

Agarose isoelectric focusing was used to demonstrate oligoclonal bands in cerebrospinal fluid (CSF) and serum from 998 consecutive neurological patients. Compared with agarose electrophoresis, agarose isoelectric focusing was slightly more sensitive, showing more (and more easily discernible) oligoclonal bands. Agarose isoelectric focusing, which has good reproducibility, revealed oligoclonal b...

Enzyme immobilization is a specific method for restricting the enzyme freedom of movement. There are strategies for the proteins multipoint immobilization by amine-terminal residues, lysine residues and carboxylic groups. In the present work, a commercial invertase of Saccharomyces cerevisiae underwent multipoint immobilization on glyoxyl-agarose, amineagarose and glutaraldehyde-agarose support...

The application of agarose in hemoperfusion is hampered by the lack of a suitable sterilization method. A technique has been developed for the crosslinking of agarose encapsulated sorbents by the reaction with 1,3-dichloro-2-propanol (DCP) under strong alkaline conditions. A twofold molar excess of DCP with respect to agarose and an equimolar amount of sodium hydroxide at a concentration of 0.3...

This purpose of this paper is to review the recent history of the use of agarose gels. Although originally confined to electrophoresis work, agarose gels have proven themselves useful to a number of disciplines in the modern world, which includes brain infusion studies for research involving the treatment of various neurological conditions, such as Parkinson's Disease. In reviewing the relevant...