The amino acid sequence of a noyel trypsin inhibitor (p20) was completed by the molecular cloning of the protein in cultured soybean ce)ls. The clone nucleotide contains an open reading frame encoding a polypeptide of 206 amino acids that shows 45-50% sequence homology to members of the Kunitz-type trypsin inhibitor fatnily. The p20 transcript is expressed in the roots, stems and )eayes ef soyb...

The objective of this study was to identify and evaluate for the first time anticoagulant fibrinogenolytic protease from Psidium guajava seed extract. extract in sodium dodecyl sulfate polyacrylamide gel electrophoresis showed protein bands high molecular weight region between 122 kDa 47 two distinct lower at ~16.6 ~14.3 kDa. content found be 1 mg/ml with specific activity 0.8 U. proteolytic su...

Sera from Indian patients with parasitologically confirmed visceral leishmaniasis were studied by immunoblot analysis in order to identify a specific pattern for Leishmania infection. A soluble extract of Leishmania donovani was used as antigen. At diagnosis the sera from patients with visceral leishmaniasis specifically recognized fractions represented by bands of 201 kDa (50% of serum samples...