During wound healing, release of ATP from platelets potentially exposes the epidermis to concentrations ofATP known to alter cellular functions mediated via changes in inositol trisphosphate (IP3) and intracellular calcium (Cai) levels. Therefore, we determined whether keratinocytes respond to ATP with a rise in Cai and IP3 and whether such increases are accompanied by a change in their prolife...

Chromaffin granules isolated from beef adrenal medulla took up C(14)-labeled catecholamines from an isotonic medium at 37 degrees C at a rate approximately 20 times the rate at 0 degrees C. The uptake was stimulated three- to five-fold by Mg(++) and adenosine triphosphate. Reserpine (1 x 10(-5)M) caused a 90 percent inhibition of uptake; ethylenediaminetetraacetic acid completely inhibited the ...

We wish to thank Karen Rutherford, Erna Rollefson, Charles Hill, and Douglas MacPherson for assistance in various phases of these experiments, and Julius Adler for the Lederberg mutants and helpful comments. The following abbreviations are used: Gal, galactose; Gal-1-P, a-D-galactose-1-phosphate; G-1-P, a-D-glucose-l-phosphate; G-6-P, glucose-6-phosphate; UDPGal, uridine diphosphogalactose; UDP...

amounts of methyl incorporation. Other nucleoside triphosphates besides adenosine triphosphate were capable of sup]x>rting the incorjioration of methyl groups from L-methionine into sRNA. The nonspecificity of the nucleoside triphosphate reaction was shown to be due to an adenosine diphosphokinase reaction and not to nucleoside tri phosphate: L-methionine nucleosidyl transferase activity. Mouse...

Protein AMPylation is a posttranslational modification (PTM) defined as the transfer of an adenosine monophosphate (AMP) from adenosine triphosphate (ATP) to a hydroxyl side-chain of a protein substrate. One recently reported AMPylator enzyme, Vibrio outer protein S (VopS), plays a role in pathogenesis by AMPylation of Rho GTPases, which disrupts crucial signaling pathways, leading to eventual ...

Glycerol kinase of Escherichia co2i has been purified and crystallized. It has a molecular weight of -3 X 106. The enzyme phosphorylates glycerol exclusively to L-a-glycerophosphate. It also catalyzes the phosphorylation of dihydroxyacetone and L-glyceraldehyde but with values of Km much higher than that for glycerol. D-Glyceraldehyde has a catalytic effect in promoting the conversion of adenos...

Soluble amino acid incorporation systems from ascites tumor cells, as well as from regenerating rat liver, incorporated arginine into the protein fraction in the absence of ribosomes. This incorporation is dependent on transfer RNA, adenosine triphosphate, and adenosine triphosphate generator, but not on guanosine triphosphate or an amino acid mixture. The reaction is inhibited by puromycin and...

amounts of methyl incorporation. Other nucleoside triphosphates besides adenosine triphosphate were capable of sup]x>rting the incorjioration of methyl groups from L-methionine into sRNA. The nonspecificity of the nucleoside triphosphate reaction was shown to be due to an adenosine diphosphokinase reaction and not to nucleoside tri phosphate: L-methionine nucleosidyl transferase activity. Mouse...

A study of the velocity with which adenosine 5’triphosphate interacts with the carriers of the respiratory chain presents an unusual opportunity to explore the mechanism of action of the energy transfer pathway (1). Previous studies of this mechanism have been limited to: (a) systems in which a portion of the pathway toward the carriers was measured by the Oi8 exchange reaction (2)) the adenosi...