The PAH1-encoded phosphatidate (PA) phosphatase in Saccharomyces cerevisiae is a pivotal enzyme that produces diacylglycerol for the synthesis of triacylglycerol (TAG) and simultaneously controls the level of PA used for phospholipid synthesis. Quantitative lipid analysis showed that the pah1Δ mutation caused a reduction in TAG mass and an elevation in the mass of phospholipids and free fatty a...

The g,~mera.I aim of ISis l)a.l~er is to show h()w t,o use locus I;o decotnpose the ii~l, erpreL:.~t, ioJl of a. phra, se iul;o two) pa..rl, s, where, oue pa, rt is I, he iittez'prel;.;:d, ion ,.ff I, he ['o,::t+ssed i tem a,n(l the or;her is sotn,:; o1:,],::,,::1; w i th wh ich this ca.n comlJine. Sup-pose, ['or exa.ml>le, we t.h(+ughl, l.ha, t; the VP ./. , a pea.oh shoutd Im iul:;rF, reted ~...

Secretion of cell wall-bound acid phosphatase by Saccharomyces cerevisiae occurs along a restricted portion of the cell surface. Acid phosphatase activity produced during derepressed synthesis on a phosphate-limited growth medium is detected with an enzymespecific stain and is localized initially to the bud portion of a dividing cell . After two to three generations of phosphate-limited growth,...

Acid phosphatases are ubiquitous enzymes that exhibit activity against a variety of substrates in vitro, although little is known about their intracellular function. In this study, we report the isolation, characterization, and partial sequence of the major acid phosphatase from soybean (Glycine max L.) root nodules. The phosphatase was purified predominantly as a heterodimer with subunits of 2...

A procedureis presentedfor the automated determination of acid phosphatase activity in biological materials from either plant or animal origin. The Technicon N-i flow diagram has been simplified, and the reagents for the measurement of enzymatically produced phenolic substanceshave been modified without lossof range or sensitivity. Both phenyiphosphateand a-naphthylphosphate, introduced by Babs...

There are a number of methods currently available for the quantitative estimation of acid and alkaline phosphatase activity in biologic materials (l-5). In all the techniques, a phosphoric acid ester serves as a substrate and calorimetric determinations are made either of the inorganic phosphate or of the organic moiety of the substrate released by enzymatic hydrolysis. A description of a new m...

Soluble epoxide hydrolase (sEH) is a bifunctional enzyme that has a C-terminus epoxide hydrolase domain and an N-terminus phosphatase domain. The endogenous substrates of epoxide hydrolase are known to be epoxyeicosatrienoic acids, but the endogenous substrates of the phosphatase activity are not well understood. In this study, to explore the substrates of sEH, we investigated the inhibition of...