GFP (green fluorescent protein)-based FRET (fluorescence resonance energy transfer) technology has facilitated the exploration of the spatio-temporal patterns of cellular signalling. While most studies have used cyan- and yellow-emitting FPs (fluorescent proteins) as FRET donors and acceptors respectively, this pair of proteins suffers from problems of pH-sensitivity and bleeding between channe...

The nitrogen K-edge spectra of aqueous proline and diglycine solutions have been measured by total electron yield near-edge X-ray absorption fine structure (NEXAFS) spectroscopy at neutral and high pH. All observed spectral features have been assigned by comparison to the recently reported spectrum of aqueous glycine and calculated spectra of isolated amino acids and hydrated amino acid cluster...

Acyl-transfer catalysed by y-glutamyltranspeptidase from bovine kidney was studied using y-Land y-D-Glu-p-nitroanilide as the donor and GlyGly as the acceptor. The transfer of the y-Glu group to GlyGly was shown to be accompanied by transfer of the y-Glu group to water (hydrolysis). The results were compared with acyl-transfer catalysed by the representative serine protease, a-chymotrypsin. The...

Published solubility data for 4,5-dihydroxyanthraquinone-2-carboxylic acid dissolved in several organic solvents of varying polarity and hydrogen-bonding character are used to calculate the Abraham model solute descriptors. Calculated descriptor values suggest that engages intramolecular hydrogen formation between two phenolic hydrogens proton acceptor sites (the lone electron pairs) on neighbo...

In this paper, the electronic eigenstates and energy spectra of single and two-interacting electrons confined in a concentric double quantum rings with a perpendicular magnetic field in the presence of         on-center donor and acceptor impurities are calculated using the exact diagonalization method. For a single electron case, the binding energy of on-center donor and acceptor impurities ar...