Urea Inhibition of UrInary N-Acetyl-j3-Dglucosaminidase Is Mixed-Type and Sensitive to Changes of pH away from the OptImum, Istv#{225}n P#{243}csi,1 Agnes Cs. Berki,2 Sarah A. Taylor,3 and Robert G. Price3 [1 Dept. of Microbiol. and Biotechnol., Lajos Kossuth Univ., P.O.B. 63, 11-4010 Debrecen, Hungary (author for correspondence; fax 36-52-3 10936); 2 Dept. of Anatomy, Univ. Med. School, H-4012...

Galactinol synthase (GS, UDP-alpha-d-galactose:1l-myo-inositol-1-O- alpha-d-galactopyranosyltransferase) is a key enzyme in the biosynthetic pathway of the raffinose family of oligosaccharides. The subcellular location of GS was studied in the parenchyma of stachyose-storing tubers of Japanese artichoke (Stachys sieboldii) by isolation of protoplasts and vacuoles. A comparison of the activities...

The objective of the study was to determine the effect of copper, zinc, cadmium and mercury ions (100, 10 and 1 mg/l) on the activity of some enzymes of carp spermatozoa. Acid phosphatase activity was proved to be relatively insensitive to zinc ions, while copper, mercury and cadmium ions effectively inhibited the activity of this enzyme. Beta-N-acetylglucosaminidase activity was sensitive only...

The products of the self-incompatibility locus of flowering plants are glycoproteins. The specificity of different alleles at this locus might be expressed through differences in either amino acid sequences or by the glycan substituents. We have investigated the numbers of N-linked glycan chains on the S-glycoproteins and obtained information on their structure by enzymic cleavage with N-glycan...

LIQUIDUS WADSLEYITE-RINGWOODITE PAIRS FRACTIONALLY CRYSTALLIZED FROM OLIVINE MELT ENCLAVES IN SHOCK MELT VEINS IN L6 CHONDRITES. A. El Goresy, M. Miyahara, E. Ohtani, T. Nagase, M. Nishijima, T. Ferroir and Ph. Gillet. Bayerisches Geoinstitut, Universität Bayreuth, 95447 Bayreuth, Germany, Email: [email protected], Graduate School of Science, Tohoku University, Sendai, 980-8758, Ja...

The "distannynes" Ar'SnSnAr' (Ar' = C6H3-2,6(C6H3-2,6-Pr(i)2)2) and ArSnSnAr (Ar = C6H3-2,6(C6H2-2,4,6-Pr(i)3)2) were examined by solid-state (119)Sn NMR and Mössbauer spectroscopy. The two compounds display substantially different spectroscopic parameters, while differing only in the absence (Ar'SnSnAr') or presence (ArSnSnAr) of a para-Pr(i) group in the flanking aryl rings of their terphenyl...

Following the rapid enzymatic transfer of an oligosaccharide (GlcNAc2Man9Glc3) from a lipid carrier to endogenous protein acceptors in membrane preparations from NIL fibroblasts, the transferred oligosaccharide chain undergoes processing. Protein-bound oligosaccharides, released from the polypeptide backbone by treatment with endo-beta-N-acetylglucosaminidase H, were analyzed by gel filtration ...

The present communication reports the occurrence of high-mannose oligosaccharides on Tamm-Horsfall glycoprotein prepared from human pooled urine. The Pronase digest of the glycoprotein was fractionated by gel filtration and a high-mannose glycopeptide species was separated from complex-type glycopeptides. When high-mannose glycopeptides were digested with endo-beta-N-acetylglucosaminidase H, fo...