Aerobic acetylene-degrading bacteria were isolated from soil samples. Two isolates were assigned to the species Rhodococcus opacus, two others to Rhodococcus ruber and Gordona sp. They were compared with known strains of aerobic acetylene-, cyanide-, or nitrile-utilizing bacteria. The acetylene hydratases of R opacus could be measured in cell-free extracts only in the presence of a strong reduc...

Acetylene, an important petrochemical raw material, is very difficult to store safely under compression because of its highly explosive nature. Here we present a porous metal-organic framework named FJI-H8, with both suitable pore space and rich open metal sites, for efficient storage of acetylene under ambient conditions. Compared with existing reports, FJI-H8 shows a record-high gravimetric a...

Vibrio succinogenes grew anaerobically at the expense of formate oxidation, with nitrous oxide (N2O) serving a terminal oxidant. N2O was quantitatively reduced to dinitrogen (N2). In the presence of 5 x 10(-2) atm (ca. 5 kPa) of acetylene (C2H2), which inhibits the reduction of N2O, growth of V. succinogenes was completely inhibited. Nitrate was reduced to nitrite or to ammonia, depending on th...

acetylene dose due to the high supply of hydrogen. Moreover, methane should not be formed, since there should not be any dissociation of acetylene on the surface. Figure 11 shows the result of an experiment in which the surface was precovered with a monolayer of hydrogen. The first dose of acetylene (0.2 X 1015 molecules of C2H2 cm-2) leads to an enormous increase in the hydrogen pressure and t...

Platinum nanoparticles functionalized with 4-ethynylphenylboronic acid pinacol ester (Pt-EPBAPE) were successfully synthesized by a simple chemical reduction procedure. Because of the formation of conjugated metal-ligand interfacial linkages, the resulting nanoparticles exhibited apparent photoluminescence arising from the nanoparticle-bound acetylene moieties that behaved analogously to diacet...

Reliable estimates of Michaelis constants (K(m)) and inhibitor constants may be obtained, in the absence of control over the amount of enzyme being added to any assay system, provided the following constraints are met. Michaelis-Menten kinetics are obeyed. Two rate measurements must be made with the same sample of enzyme: at low and high substrate concentration for determining K(m) or minus and...

Nitrogen fixation, as measured by acetylene reduct~on, has been detected to be associated with various hermatypic corals. Experiments were carried out on the massive coral Favia f a v u s both in situ and in the laboratory. Nitrogen fixation activity was found to be light dependent and fully inhibited by 5 X 10-6 M DCMU [3-(3,4-dichloropheny1)-1,l-dimethylurea Addition of glucose restored nitro...

To date diamond films grown with the combustion technique have used either acetylene or, rarely, ethylene as the fuel. However, there are barriers to large scale commercialization of the combustion technique using either fuel. For example, acetylene is relatively expensive and difficult to handle, while the use of ethylene gives relatively low growth rates. In this letter we propose replacing a...