Here we examined the impact of a commonly employed method used to measure nitrogen fixation, the acetylene reduction assay (ARA), on a marine sediment community. Historically, the ARA technique has been broadly employed for its ease of use, in spite of numerous known artifacts. To gauge the severity of these effects in a natural environment, we employed high-throughput 16S rRNA gene sequencing ...

Since the discovery of acetylene and benzene in protoplanetary nebulae under powerful ultraviolet ionizing radiation, efforts have been made to investigate the polymerization of ionized acetylene. Here we report the efficient formation of benzene ions within gas-phase ionized acetylene clusters (C2H2)n+ with n = 3-60. The results from experiments, which use mass-selected ion mobility techniques...

Recently, many scientists have focused on the development of green industrial technology. However, the process of synthesizing vinyl chloride faces the problem of Hg pollution. Via a novel approach, we used two elements Mo and Ti to prepare an inexpensive and green binary transition metal nitride (BTMN) as the active ingredient in a catalyst with nano-sized particles and an excellent degree of ...

We report a versatile divergent methodology to construct dendrimers from a tetrafunctional core, utilizing the robust copper(I) catalyzed alkyne-azide cycloaddition (CuAAC, "click") reaction for both dendrimer synthesis and post-synthesis functionalization. Dendrimers of generations 1-3 with 8-32 protected or free OH and acetylene surface groups, were synthesized using building blocks that incl...

An investigation was made of various factors, both experimental and physiological, which influenced the formation of hydrogen gas by the heterocystous cyanobacterium Anabaena cylindrica B629 when incubated in both argon and air. A. cylindrica B629 produces hydrogen in air in the presence of carbon monoxide, acetylene, or both, with a short lag period. The rate of production in air at optimal co...

The rate and capacity for chloroform (CF) and trichloroethylene (TCE) transformation by a mixed methanotrophic culture of resting cells (no exogenous energy source) and formate-fed cells were measured. As reported previously for TCE, formate addition resulted in an increased CF transformation rate (0.35 day-1 for resting cells and 1.5 day-1 for formate-fed cells) and transformation capacity (0....

In this paper, we report a study on the reactivity of the phenylium ion with acetylene, by measuring product yield as a function of pressure and temperature using mass-selected ion mobility mass spectrometry. The reactivity is dominated by a rapid sequential addition of acetylene to form covalently bonded C8H7(+) and C10H9(+) ions with an overall rate coefficient of 7-5 × 10(-10) cm(3) s(-1), i...

The involvement of epiphytic microorganisms in nitrogen fixation was investigated in a shallow freshwater pond near Ithaca, N.Y. The acetylene reduction technique was used to follow diel and seasonal cycles of nitrogen fixation by epiphytes of Myriophyllum spicatum. Acetylene-reducing activity was maximal between noon and 6 p.m., but substantial levels of activity relative to daytime rates cont...

Although the ethyl iodide method of Starr and Gamble (1) and the acetylene method of Grollman (2) give useful results in the determination of the cardiac output in man, they require special apparatus and a time consuming technique not easy to master. It appeared that if the determination of the cardiac output is to become a practical procedure for the clinic a further simplification of methods ...

Acetylene is a colorless gas commonly used for welding. It acts mainly as a simple asphyxiant. In this paper, however, we present a patient who developed a severe interstitial pneumonitis after acetylene exposure during aluminum welding. A 44-year old man was welding with acetylene, argon and aluminum electrode sticks in a non-ventilated aluminum tank for 2 h. Four hours after welding dyspnea a...