In isolated hepatocytes prepared from unfed neonatal chicks, stimulation of fatty acid synthesis by fructose or dihydroxyacetone required acetate or octanoate in the medium, suggesting that the stimulatory effect of these compounds required the enhanced production of intramitochondrial acetyl coenzyme A. Stimulation of fatty acid synthesis by lactate or pyruvate did not require a supplementary ...

Fasted (48 h) rats were killed at 0, 2, 4, 6, 8, 12, 16, 20 and 24 h after they were refed on a high-carbohydrate diet. An increase in the maximal activity and quantity of cystolic acetyl-CoA carboxylase was found in liver of refed rats after a lag time of about 8 h. The increased quantity of cytosolic enzyme was attributable primarily to mobilization of mitochondrial storage forms and not to s...

Acetyl coenzyme A (CoA) carboxylase activity of whole tissue homogenates and chloroplast preparations was analyzed as the acetyl-CoA-dependent incorporation of [(14)C]bicarbonate into an acid-stable product. The absolute requirement for ATP and MgCl(2), the complete inhibition with avidin, and end-product analysis were consistent with the presence of acetyl-CoA carboxylase activity. Little diff...

The occurrence of multiple herbicide resistant weeds has increased considerably in glyphosate-resistant soybean fields Brazil; however, the mechanisms governing this resistance have not been studied. In its study, target-site and nontarget-site were characterized an Eleusine indica population (R-15) with to acetyl-CoA carboxylase (ACCase) inhibitors, glyphosate, imazamox, paraquat. Absorption t...

Studies presented here, with the criterion of the degree of binding of the biotin derivatives obtained from acetyl coenzyme A carboxylase to avidin, show that these compounds have an intact ureido ring, eliminating the diamine derivative as an intermediate in the carboxylation. With the use of carboxyl-14C-biotin acetyland propionyl-CoA carboxylases, it has been possible to show both in vivo an...

The effects of two hypolipidemic agents, 2-methyl-2-[p(1,2,3,4-tetrahydro-l-naphthyl)-phenoxy]propionate and ethyl 2-(p-chlorophenyl)-2-methylpropionate, on the activity of a highly purified preparation of acetyl coenxyme A carboxylase, obtained from chicken livers, were studied. Both compounds are inhibitors of acetyl-CoA carboxylase, but they have no inhibitory effect on a number of other enz...

Acetyl-coenzyme A (acetyl-CoA) formed within the plastid is the precursor for the biosynthesis of fatty acids and, through them, a range of important biomolecules. The source of acetyl-CoA in the plastid is not known, but two enzymes are thought to be involved: acetyl-CoA synthetase and plastidic pyruvate dehydrogenase. To determine the importance of these two enzymes in synthesizing acetyl-CoA...