Early region E3 of adenovirus 2 encodes a glycoprotein, E3-gp25K, that is a good model with which to study structure-function relationships in transmembrane glycoproteins. We have determined the structures of the oligosaccharides linked to E3-gp25K. The oligosaccharides were labeled with [2-(3)H]mannose in adenovirus 2-early infected KB cells for 5.5h (pulse) or for 5.5 h followed by a 3-h chas...

The human pathogen Candida albicans encodes at least three putative two-component histidine kinase signal transduction proteins, including Chk1p and a response regulator protein (Cssk1p). Strains deleted in CHK1 are avirulent in a murine model of hematogenously disseminated disease. The specific function of Chk1p has not been established, but hyphae of the chk1 mutant exhibit extensive floccula...

A previous study (vijay, I. K., Perdew, G. H., and Lewis, D. E. (1980) J. BioL Chem. 255, 11210-11220) indicated that incubation of a membrane preparation from the lactating bovine mammary tissue with UDPISH]GlcNAc and GDP-[’“C]Man synthesized a series of lipid-linked saccharides that appeared to be related to one another as precursor products in the biosynthesis of asparagine-linked glycoprote...

Phosphomannosyl residues on acid hydrolases serve as recognition markers which target these enzymes to lysosomes. We have found that the oligosaccharide units of newly synthesized beta-glucuronidase contain phosphate residues in diester linkage between mannose and alpha-linked N-acetylglucosamine residues (Tabas, I., and Kornfeld, S. (1980) J. Biol. Chem. 255, 6633--6639). To obtain larger amou...

IT has long been known that the polysaccharide chitin when completely hydrolysed yields glucosamine and acetic acid; in recent years it has indeed been shown conclusively that the basic unit of the polysaccharide is N-acetylglucosamine. The evidence for the latter statement rests, firstly, on the discovery by Karrer & Hofmann [1929] ofan enzyme in Helixpomatiawhich is able to hydrolyse chitin t...

In the course of a study of pollen wall development in the Gramineae two interesting relationships were noted: the locule wall and tapetal cells were bordered by spheroids which were similar chemically (resistant to acetolysis) and morphologically to the pollen exine. The pollen exine as well as the wall of the spheroids was perforated by submicroscopic channels. In the mature anther the sphero...

A novel reaction was explored in which synthetic platelet-activating factor, l-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (AGEPC), upon treatment with 1 N NaOH in methanol at 6OoC for 20 min, sequentially released the acetyl group, then the choline moiety with concomitant formation of the monomethyl ester of 1-0-alkyl-glycero-phosphoric acid. A mechanism is proposed in which a transient cycli...