Liver cancer is a serious threat to public health and has fairly complicated pathogenesis. Therefore, the identification of key genes and pathways is of much importance for clarifying molecular mechanism of hepatocellular carcinoma (HCC) initiation and progression. HCC-associated gene expression dataset was downloaded from Gene Expression Omnibus database. Statistical software R was used for si...

This study was designed to determine whether methyl-b-cyclodextrin (MCD) can substitute for albumin in incubation medium for neonatal swine adipose tissue explants, or whether MCD affects metabolism and cytokine expression. Subcutaneous adipose tissue explants (1006 10 mg) were prepared from 21-day-old pigs. Explants were incubated in medium 199 supplemented with 25 mM HEPES, 1.0 nM insulin at ...

Acetoacetylated (AcAc) and acetylated (Ac) low density lipoproteins (LDL) are rapidly cleared from the plasma (t1/2 approximately equal to 1 min). Because macrophages, Kupffer cells, and to a lesser extent, endothelial cells metabolize these modified lipoproteins in vitro, it was of interest to determine whether endothelial cells or macrophages could be responsible for the in vivo uptake of the...

Adipose tissue is one of the major sites for fatty acid synthesis and lipid storage. We generated adipose (fat)-specific ACC1 knockout (FACC1KO) mice using the aP2-Cre/loxP system. FACC1KO mice showed prenatal growth retardation; after weaning, however, their weight gain was comparable to that of wild-type (WT) mice on a normal diet. Under lipogenic conditions of fasting/re-feeding a fat-free d...

SUBRAMANIAM, KAMALA. Radio Resource Management in UMTS-WCDMA Systems. (Under the direction of Professor Arne A. Nilsson). Universal Mobile Telecommunications System (UMTS) is a Third Generation (3G) cellular technology representing an evolution of a heterogenous mix of services and increased data speeds from today’s second generation mobile networks. UMTS uses Wideband Code Division Multiple Ac...

CYP4A6/P450 IV rabbit AACT AGGGCA A AGTTGA [1] CYP4A1/P450 IV rat AACT AGGGTA A AGTTCA [2] L-fatty acid binding protein rat ATAT AGGCCA T AGGTCA* [3] 3-hydroxy-3-methyl-glutaryl-CoA-synthase rat AACT GGGCCA A AGGTCT* [4] Enoyl-CoA-hydratase rat ATGT AGGTAA T AGTTCA* [1] Malic enzyme rat TTCT GGGTCA A AGTTGA [5] Phosphoenolpyruvate carboxikinase rat AACT GGGATA A AGGTCT [6] Phosphoenolpyruvate c...

During starvation ketone bodies, acetoacetate (AcAc), 3-hydroxybntyrate (fl-OHB) and acetone accumulate in the body fluids. AcAc and /3-OHB are synthesized in the liver primarily from the partial oxidation of long-chain fatty acids. They are released into the blood as short-chain fatty acids, dissociate to become water-soluble anions and are distributed at different concentrations in the water ...

Acetyl-CoA carboxylase 1 (ACC1) catalyzes the formation of malonyl-CoA, the C2 donor for de novo synthesis of long-chain fatty acids. We have identified 64 exons, including 7 alternatively spliced minor exons (1A, 1B, 1C, 3, 5A', 5A, and 5B) in human ACC1 gene ( approximately 330 kb). The gene is regulated by three promoters (PI, PII, and PIII), which are located upstream of exons 1, 2, and 5A,...

Identification of reference genes with stable levels of gene expression is an important prerequisite for obtaining reliable results in analysis of gene expression data using quantitative real time PCR (RT-qPCR). Since the underlying assumption of reference genes is that expressed at the exact same level in all sample types, in this study, we evaluated the expression stability of nine most commo...

The external electroluminescence (EL) quantum efficiency (QEEL) of a polymer light-emitting diode (PLED) can be affected by the following four factors: a) charge balance, b) the efficiency of producing singlet excitons, c) photoluminescence quantum efficiency (QEPL), and d) the output coupling effect. The QEPL can approach unity and the efficiency of producing singlet excitons can be high in lo...