PURPOSE The purpose of this study was to quantify accommodation-induced changes in the spherical aberration of cynomolgus monkey lenses. METHODS Twenty-four lenses from 20 cynomolgus monkeys (Macaca fascicularis; 4.4-16.0 years of age; postmortem time 13.5 ± 13.0 hours) were mounted in a lens stretcher. Lens spherical aberration was measured in the unstretched (accommodated) and stretched (re...

The main resolution limits in high-performance (S)TEM are due to the imperfections or ‘aberrations’ of the electron lenses. Correction of these aberrations requires the ability to quickly and accurately measure lens aberrations to align or ‘tune’ an aberration-corrector. The conventional method to measure aberrations in the TEM is to record a series of bright field (BF) images in a Zemlin table...

We examine wave-front distortion caused by high-power lasers on transmissive optics using a Shack-Hartmann wave-front sensor. The coupling coefficient for a thermally aberrated Gaussian beam to the TEM(00) mode of a cavity was determined as a function of magnitude of the thermally induced aberration. One wave of thermally induced phase aberration between the Gaussian intensity peak and the 1/e(...

Malignant solid tumors are commonly characterized by a large number of complex structural and numerical chromosomal alterations, which often reflect the level of genomic instability and can be associated with disease progression. The aim of this study was to evaluate whether chromosomes that harbor primary aberrations have a higher susceptibility to accumulate further alterations. We used spect...

Electron holography has been shown to allow a posteriori aberration correction. Therefore, an aberration corrector in the transmission electron microscope does not seem to be needed with electron holography to achieve atomic lateral resolution. However, to reach a signal resolution sufficient for detecting single light atoms and very small interatomic fields, the aberration corrector has turned...

A wavefront sensorless adaptive optics technique was combined with a custom-made multiphoton microscope to correct for specimen-induced aberrations. A liquid-crystal-on-silicon (LCoS) modulator was used to systematically generate Zernike modes during image recording. The performance of the instrument was evaluated in samples providing different nonlinear signals and the benefit of correcting hi...

Over the past decade, a number of information forensic techniques have been developed to identify digital image manipulation and falsifications. However, recent research has shown that an intelligent forger can use antiforensic countermeasures to disguise their forgeries. In this paper, an anti-forensic technique is proposed to falsify the lateral chromatic aberration present in a digital image...

It is known that the depth of focus (DOF) of the human eye can be affected by the higher order aberrations. We estimated the optimal combinations of primary and secondary Zernike spherical aberration to expand the DOF and evaluated their efficiency in real eyes using an adaptive optics system. The ratio between increased DOF and loss of visual acuity was used as the performance indicator. The r...

Spherical aberration arising from deviations of the thickness of an optical disc substrate from a nominal value can be compensated to a great extent by illuminating the scanning objective lens with a slightly convergent or divergent beam. The optimum conjugate change and the amount and type of residual aberration are calculated analytically for an objective lens that satisfies Abbe's sine condi...

PURPOSE To develop a corneal model to better explain how refractive surgery procedures induce spherical aberration. SETTING Department of Ophthalmology and Center for Visual Science, University of Rochester, Rochester, New York, USA. METHODS The preoperative cornea was modeled as a rotationally symmetric surface with various radii of curvature and asphericities. The postoperative cornea was...