نتایج جستجو برای: aav base vector

تعداد نتایج: 451713  

2018
Pasqualina Colella Giuseppe Ronzitti Federico Mingozzi

In recent years, the number of clinical trials in which adeno-associated virus (AAV) vectors have been used for in vivo gene transfer has steadily increased. The excellent safety profile, together with the high efficiency of transduction of a broad range of target tissues, has established AAV vectors as the platform of choice for in vivo gene therapy. Successful application of the AAV technolog...

Journal: :Bioscience, biotechnology, and biochemistry 2009
Keisuke Sato Shiori Date Yumi Aoyagi Yoshiyuki Kasahara Akihiko Nawa Hiroaki Mizukami Shizu Hidema Keiya Ozawa Katsuhiko Nishimori

We developed the AAV-Oxtr-IRES-Venus vector to rescue the oxytocin receptor (Oxtr) gene functionally at restricted regions in the brains of Oxtr knockout mice. First we chose human eIF4G gene-derived IRES to co-express Venus, a fluorescent marker gene, with Oxtr. With selected human eIF4G IRES, we constructed the AAV-Oxtr-IRES-Venus vector, and it caused expression of the Venus gene in the brai...

Journal: :Clinical microbiology reviews 2008
Shyam Daya Kenneth I Berns

SUMMARY The unique life cycle of adeno-associated virus (AAV) and its ability to infect both nondividing and dividing cells with persistent expression have made it an attractive vector. An additional attractive feature of the wild-type virus is the lack of apparent pathogenicity. Gene transfer studies using AAV have shown significant progress at the level of animal models; clinical trials have ...

2011
Zejing Wang Stephen J. Tapscott Jeffrey S. Chamberlain Rainer Storb

Adeno-associated viral (AAV) vector-mediated gene replacement for the treatment of muscular dystrophy represents a promising therapeutic strategy in modern medicine. One major obstacle in using AAV vectors for in vivo gene delivery is the development of host immune responses to the viral capsid protein and transgene products as evidenced in animal models and human trials for a range of genetic ...

Journal: :Journal of virology 2005
Daniel G Miller Grant D Trobridge Lisa M Petek Michael A Jacobs Rajinder Kaul David W Russell

The integration sites of viral vectors used in human gene therapy can have important consequences for safety and efficacy. However, an extensive evaluation of adeno-associated virus (AAV) vector integration sites has not been completed, despite the ongoing use of AAV vectors in clinical trials. Here we have used a shuttle vector system to isolate and analyze 977 unique AAV vector-chromosome int...

2009
Ryan R. Klimczak James T. Koerber Deniz Dalkara John G. Flannery David V. Schaffer

BACKGROUND The pathologies of numerous retinal degenerative diseases can be attributed to a multitude of genetic factors, and individualized treatment options for afflicted patients are limited and cost-inefficient. In light of the shared neurodegenerative phenotype among these disorders, a safe and broad-based neuroprotective approach would be desirable to overcome these obstacles. As a result...

2017
Lisa A. Santry Joelle C. Ingrao Darrick L. Yu Jondavid G. de Jong Laura P. van Lieshout Geoffrey A. Wood Sarah K. Wootton

BACKGROUND Targeted delivery of gene therapy vectors to the mouse respiratory tract is often performed via intranasal or intratracheal administration; however, there can be a great deal of variability between these methods, which could potentially influence experimental results. Improving the accuracy and precision of lung delivery will not only reduce the number of animals required to detect s...

Journal: :Acta biochimica et biophysica Sinica 2004
Yi-Gang Wang Jin-Hui Wang Yan-Hong Zhang Qing Gu Xin-Yuan Liu

Telomerase activity is a wide tumor marker. Human telomerase reverse transcriptase (hTERT), the catalytic subunit of the telomerase, is transcriptionally upregulated exclusively in about 90% of cancer cells. In this study, we constructed a novel adeno-associated virus (AAV) vector containing the human interferon-beta (hIFN-beta) gene under the control of hTERT promoter (AAV-hTERT-hIFN-beta) and...

Journal: :Postdoc journal : a journal of postdoctoral research and postdoctoral affairs 2015
Sara E Gombash

Gene therapy to the gastrointestinal tract has remarkable potential for treating gastrointestinal disorders that currently lack effective treatments. Adeno-associated viral vectors (AAVs) have been extensively applied to the central nervous system, and have repeatedly demonstrated safety and efficacy in animal models. The enteric nervous system (ENS) represents a vast collection of neurons and ...

2013
Chukai Huang Ling-Ping Cen Lifang Liu Simone G. Leaver Alan R. Harvey Qi Cui Chi Pui Pang Mingzhi Zhang

PURPOSE To examine whether adeno-associated virus (AAV) vector-mediated overexpression of growth-associated protein-43 (GAP-43) has protective or deleterious effects on retinal ganglion cell (RGC) survival in laser-induced chronic intraocular pressure (IOP) elevation injury. METHODS Adult Fischer 344 rats received unilateral intravitreal injection of either normal saline, AAV-green fluorescen...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید