Background: Aflatoxin B1 is a secondary toxic metabolite produced by several aspergillus species. This study was conducted to determine the presence of aflatoxin in tomato paste samples. Methods: A total of 30 tomato pastes were analyzed for Aflatoxin B1 contamination via HPLC-FLD. Analyte extraction was done by dispersive liquid-liquid microextraction. Results: The limit of detection (LOD) and...

We have constructed derivatives of Streptomyces coelicolor M145 as hosts for the heterologous expression of secondary metabolite gene clusters. To remove potentially competitive sinks of carbon and nitrogen, and to provide a host devoid of antibiotic activity, we deleted four endogenous secondary metabolite gene clusters from S. coelicolor M145--those for actinorhodin, prodiginine, CPK and CDA ...

Iguratimod is a new synthetic disease-modifying antirheumatic drug intended to treat patients with rheumatoid arthritis. A new method using recombinant human CYP450s yeast cells containing c-DNA expressed P450s was applied to identify the metabolic pathways of iguratimod and to prepare its metabolite. The metabolite was isolated, and its structure was identified by quadrupole time-of-flight-mas...

background: elicitors can trigger defense responses and activate specific genes involved in secondary metabolite biosynthesis. objective: for understanding sa-treatment signaling pathway we determined defense-related compounds and enzymes in s. marianum hairy root cultures. methods: 28 day old hairy roots were treated by salicylic acid (6 mg/50 ml culture) for different times (24, 48, 72, 96 an...

Sericostoma pauciflorum Stocks ex Wight(Family Boraginaceae) used against cancer, diabetes and known to be health promoter. Callus cultures have been established from the stem explants on Murashige and Skoog (MS) medium supplemented with 1.5 mg/L concentration of different growth hormone viz. kinetin (Kn), indole 3-acetic acid (IAA) and indole 3-butyric acid (IBA). At 6 weeks of age, these call...