The fungus Venturia nashicola is the causal agent of scab on Asian pears. For the rapid and reliable identification as well as sensitive detection of V. nashicola, a PCR-based technique was developed. DNA fingerprints of three closely related species, V. nashicola, V. pirina, and V. inaequalis, were obtained by random amplified polymorphic DNA (RAPD) analysis. Two RAPD markers specific to V. na...

Bacillary dysentery, or shigellosis, is a disease of humans in which the colonic epithelium is invaded by bacteria and subjected to inflammatory destruction. The aim of this study was to develop a polymerase chain reaction(PCR) test for detection of virulent Shigella spp.. For this purpose, the primers were designed to amplify a 526-bp internal region of the Shigella spp. icsA gene, which encod...

background toxoplasmosis is a widespread disease in humans and many other species of warm-blooded animals. among livestock animals, sheep and goat are more widely infected by toxoplasma gondii. this parasite is a major cause of abortion, with significant economic losses for sheep and goat breeders. objectives the polymerase chain reaction (pcr) method was employed to detect of the t. gondii dna...

background: cryptosporidiosis is one of the most important parasitic infections in human and animals. this study was designed for survey on the prevalence of cryptosporidium infection in farms of ilam, west of iran, using parasitology method and genotyping by nested pcr-rflp. methods : fecal samples of 217 cattle were collected fresh and directly from the rectum of cattle. all of the samples we...

Bovine theileriosis is important disease in tropical and subtropical areas with great economic losses in livestock husbandry in Iran. The aim of study was to assess the prevalence of Theileria annulata infection in cattle and Hyalomma species of Kermanshah Province, Iran. A number of 138 blood samples were randomly taken from examined cattle. The genomic DNA was extracted and PCR was performed ...

background: acanthamoeba keratitis develops by pathogenic acanthamoeba such as a. pal­es­tinen­sis . indeed this species is one of the known causative agents of amoebic keratitis in iran. mannose binding protein (mbp) is the main pathogenicity factors for developing this sight threatening disease. we aimed to characterize mbp gene in pathogenic acanthamoeba isolates such as a. palestinensis . m...

Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is one of the most important pest of vegetable and fruit crops. This polyphagous pest has a range of natural enemies including the parasitoid Eretmocerus mundus (Merect) (Hymenoptera: Aphelinidae). To determine the molecular profile and taxonomic status of Iranian isolates of E. mundus, parasitized B. tabaci samples were collected from cotton...

background: the heartworm disease is an infectious disease of dogs with dirofilaria immitis combined with cardiovascular and circulatory abnormalities. the heartworm disease can become a serious health risk when associated with a severe infection. in this study, a male, 8 year-old dog that died suddenly was necropsied and all tissues were examined grossly. methods: major organs including heart,...

The 680 bp fragment of the COI gene was employed for DNA barcodes millipede genus Tylopus in Vietnam. A total 22 samples representing 14 morphological species were analyzed. K2P genetic divergence between ranges from 12.2% to 18.9% with a mean 15 ± 1%. intraspecific divergences are slightly different species, 3% 5%. AGBD analysis and phylogenetic trees also support species. It is suggested have...

We report the interaction between a human centromere antigen and an alphoid DNA, a human centromeric satellite DNA, which consists of 170-bp repeating units. A cloned alphoid DNA fragment incubated with a HeLa cell nuclear extract is selectively immunoprecipitated by the anticentromere sera from scleroderma patients. Immunoprecipitation of the DNA made by primer extension defines the 17-bp segm...