The interaction of lipoprotein lipase (LPL) with heparan sulfate proteoglycans plays an important role in the metabolism and catalytic function of the enzyme. We have used site-directed mutagenesis to replace the basic residues contained in a discontinuous charge cluster (residues Lys 321, Arg 405, Arg 407, Lys 409, Lys 415, and Lys 416) of avian LPL with asparagine. The mutant proteins were ex...

We present data on the stellar kinematics of the brightest ellipticals in HCG 62, HCG 68, NGC 2300 groups. Moreover, we report on ASCA GIS as well as optical observations of the early-type member of the pair K 416 (NGC 5480/5481) similar, in many respects, to the dominant pair in the NGC 2300 group. The kinematics of HCG 62a/b and HCG 68a/b indicate that they are not interacting pairs. HCG 62a ...

Src and Src-family protein-tyrosine kinases are regulatory proteins that play key roles in cell differentiation, motility, proliferation, and survival. The initially described phosphorylation sites of Src include an activating phosphotyrosine 416 that results from autophosphorylation, and an inhibiting phosphotyrosine 527 that results from phosphorylation by C-terminal Src kinase (Csk) and Csk ...