Reaction conditions for the reducing-end-specific derivatization of cellulose substrates with the fluorogenic compound, anthranilic acid, have been established. Hydrolysis of fluorescence-labelled celluloses by cellobiohydrolase Cel7A from Trichoderma reesei was consistent with the active-site titration kinetics (burst kinetics), which allowed the quantification of the processivity of the enzym...

Degradation of cotton cellulose by Trichoderma reesei endoglucanase I (EGI) and cellobiohydrolase II (CBHII) was investigated by analyzing the insoluble cellulose fragments remaining after enzymatic hydrolysis. Changes in the molecular-size distribution of cellulose after attack by EGI, alone and in combination with CBHII, were determined by size exclusion chromatography of the tricarbanilate d...

in this paper detection of endoglucanase gene and protein profile belonging to two fungal species, penicillium oxalicum 1sms and trichodermaviride156ms with high cellulotic activity was investigated. fungi isolates were cultured on inducer cmc medium and then released sugar and protein were assayed every three days for a month using arsenate molybdate reagent and bradford method, respectively. ...

Cellulases are enzymes used in the hydrolysis of lignocellulosic residues for second-generation (2G) bioethanol production, low availability market, and high-cost impacts 40% total cost 2G production. Therefore, it is necessary to look sustainable alternatives its mainly produced by Aspergillus niger, their activity can be affected nitrogen concentration, use surfactants, carbon source, among o...

BACKGROUND Bipolaris sorokiniana is a filamentous fungus that causes spot blotch disease in cereals like wheat and has severe economic consequences. However, information on the identities and role of the cell wall-degrading enzymes (CWDE) in B. sorokiniana is very limited. Several fungi produce CWDE like glycosyl hydrolases (GHs) that help in host cell invasion. To understand the role of these ...

Cellulase is a group of enzymes (endoglucanase, exoglucanase and betaglucosidase) required for cellulosic feedstock hydrolysis during bioethanol production. The use of recombinant cellulase is a strategy to reduce the enzyme cost. In this context, the present work describes the construction of a cellulase expression vector (pEglABglA), which allowed constitutive co-expression of endoglucanase A...

This article reports the characterization of the Clostridium thermocellum SdbA protein thought to anchor the cellulosome to the bacterial cell surface. The NH2-terminal region of SdbA consists of a cohesin domain which specifically binds the dockerin domain of the cellulosomal scaffolding protein CipA. The COOH-terminal region consists of a triplicated segment, termed SLH repeats, which is pres...

Two kinds of xyloglucans were obtained from the cell walls of suspension-cultured soybean cells together with an additional one from the culture medium. They had almost the same properties, i.e., sugar composition, [α]d value, and iodine staining spectrum. The molecular weights of the two kinds of wall xyloglucans were 180,000 and 60,000, whereas that of the extracellular xyloglucan shifted fro...

A cross-linked mutant endoglucanase II was prepared for enzymatic polymerization to cellulose. The cross-linked enzyme is composed of three mutant enzymes showing polymerization activity. A characteristic feature of the polymerization with this cross-linked enzyme is formation of cellulose fibrils in contrast to plate-like crystals obtained by using a free enzyme.