An homogenate of rat liver which was capable of converting 2-3H-lanosta-8,24-dien-3fl-ol to 2-3H-cholest-5-en-3/3-ol failed to convert 2-3H-9, 19-cyclolanost-24-en-30-01 to 2-3Hcholest-5-en-3/3-01. However, both substrates underwent reduction at C-24,25. 2-3H-9, 19-Cyclolanost-24-en-3/?-01 was not converted to 2-3H-lanosta-8,24-dien-3P-ol. Both the presence and biosynthesis (from 2-14C-mevalona...

The phytochemical study of Dorstenia arifolia Lam. (Moraceae) has led to the identification of 18 triterpenes esterified by fatty acids, five triterpenes without esterification, 12 triterpenes esterified by acetic acid, together with a known furanocoumarin: α-amyrin (1), β-amyrin (2) α-amyrin acetate (3) β-amyrin acetate (4), α-amyrin octanoate (5), β-amyrin octanoate (6), α-amyrin decanoate (7...

Objective: Microbial catalysis is used in the commercial production of many bioactive steroids. Solid phase microbial fermentation of anabolic steroid, dihydrotestosterone (DHT, 1), was carried out with ascomycete fungal strain Fusarium oxysporum (NRRL-1392). Methods: Sabouraud-4% glucose-agar was used to cultivate the fungal cultures as solid phase medium. Substrate 1 was incubated with Fusari...

3-Ketosteroid-∆1-dehydrogenase (KstD), a key enzyme in microbial steroid catabolism, catalyzes the trans-axial elimination of the C1 and C2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3-ketosteroids, and it was usually used to transform androst-4-ene-3,17-dione (AD) to produce androsta-1,4-diene-3,17-dione. Here, the KstD from Gordonia neofelifaecis was expressed efficien...

Five new highly oxygenated lanostane-type triterpenoids [ganoderic acid GS-1 (1), ganoderic acid GS-2 (2), ganoderic acid GS-3 (3), 20(21)-dehydrolucidenic acid N (4) and 20-hydroxylucidenic acid A (5)] were isolated from the fruiting body of Ganoderma sinense, together with known compounds including 6 triterpenoids and 3 sterols. The structures of the new triterpenoids determined by spectrosco...

Two new organic hybrid thiogermanates [Ni(dien)2](H2dien)Ge2S6 (1, dien = diethylenetriamine) and [Ni(teta)2]2Ge4S10 (2, teta = triethylenetetramine) were synthesized under solvothermal conditions. 1 contains a [Ni(dien)2] complex cation, a protonated dien molecule and a [Ge2S6]4− dimeric anion, while 2 consists of discrete [Ge4S10]4− adamantane-like cluster anions with [Ni(teta)2] complex cati...

The screening of testosterone misuse in the doping control field is normally performed by the measurement of the ratio between the concentrations of testosterone and epitestosterone excreted as glucuronides (T/E). Despite the satisfactory results obtained with this approach, the measurement of T/E presents some limitations like the long-term detection of oral testosterone administration. Recent...