6'-(4-Chlorophenyl)-spiro[cyclohexane-1,2'-thieno[3,2-d][1,3] oxazin]-4'(1'H)-one (1) was synthesized and used as a starting material for the synthesis of a novel series of spiro compounds having biologically active sulfonamide 2a-e and 3'-(4-acetylphenyl)-6'- (4-chlorophenyl)-1'H-spiro[cyclohexane-1,2'-thieno[3,2-d] pyrimidine-4'(3'H)-one (3). Compound 2a was used as a key intermediate for the...

N1-Substituted-5-amino-4-cyanopyrazoles were cyanoacetylated with a mixture of cyanoacetic acid and acetic anhydride. Cyclization with POCl3 gave 4-chloro-pyrazolo[3,4-d]pyrimidine derivatives. From the reaction with hydrazine and arylhydrazines, the hydrazinyls and their oxidized forms, the azo products, were obtained. The structure of the compounds obtained has been confirmed by 1H and 13C NM...

The recent development of a new series of carcinostatic purine analogs, the pyrazolo(3,4-d)pyrimidines (5), has led to their study as substrates and inhibitors of certain purine oxidation systems in vitro (1,4). In the course of these studies, it was observed that purified xanthine oxidase incubated in vitro with 4-aminopyrazolo(3,4-d)pyrimidine (pyrazoloadenine) resulted in its oxidation to 4a...

BACKGROUND Some novel substituted pyrazolone, pyrazolo[3,4-c]pyridazine, pyrazolo[3,4-d]pyrimidine, pyrazolo[3,4-d]thiazolo[3,2-a]pyrimidinone, thieno[3,2-c]pyrazole and pyrazolo[3',4':4,5]thieno[2,3-d]pyrimidine derivatives have been reported to possess various pharmacological activities like antimicrobial, antitumor and anti-inflammatory. RESULTS A novel series of azoles and azines were des...

The recent development of a new series of carcinostatic purine analogs, the pyrazolo(3,4-d)pyrimidines (5), has led to their study as substrates and inhibitors of certain purine oxidation systems in vitro (1,4). In the course of these studies, it was observed that purified xanthine oxidase incubated in vitro with 4-aminopyrazolo(3,4-d)pyrimidine (pyrazoloadenine) resulted in its oxidation to 4a...