In vitro cell-based models of lung cancer are frequently employed to study invasion and the mechanisms behind metastasis. However, these models often study only one cell type with two-dimensional (2D) monolayer cell cultures, which do not accurately reflect the complexity of inflammation in vivo. Here, a three-dimensional (3D) cell co-culture collagen gel model was employed, containing human lu...

The PromoCell Cancer Stem Cell Medium has been designed to meet your requirements for the extended serial 3D tumorsphere culture. This Cancer Stem Cell Medium supports the most commonly used cancer stem cell lines in tumorsphere/mammosphere culture. In contrast to the current adherent 2D culture of cancer cells, this type of 3D culture selectively exploits inherent biologic features of Cancer S...

The PromoCell Cancer Stem Cell Medium has been designed to meet your requirements for the extended serial 3D tumorsphere culture. This Cancer Stem Cell Medium supports the most commonly used cancer stem cell lines in tumorspheres/ mammospheres culture. In contrast to the current adherent 2D culture of cancer cells, this type of 3D culture selectively exploits inherent biologic features of CSC, ...

The low efficiency of differentiation into male germ cell (GC)-like cells and haploid germ cells from human embryonic stem cells (hESCs) reflects the culture method employed in the two-dimensional (2D)-microenvironment. In this study, we applied a three-step media and calcium alginate-based 3D-culture system for enhancing the differentiation of hESCs into male germ stem cell (GSC)-like cells an...

BACKGROUND Collagen-based three-dimensional (3D) in vitro systems have been introduced to study the physiological states of cells. As a biomolecule, collagen is usually extracted from terrestrial animals whilst aquatic animals like squid contain large amounts of collagen. METHODS In order to make effective use of marine organisms, we selected Persian Gulf squid in 2015 to extract the required...