Teflon spaghetti tubing was placed over the metal needles of the dispensing head of the Accu-Drop multidropper, thus preventing the delivery of multiple drops. A metal plate was drilled so that 25 cartridges could be positioned to inoculate simultaneously 25 compartments in a divided petri dish.

In the field of microwave hyperthermia, the use of minimally invasive applicators is recognized as a very promising technique for the treatment of small tumours because a very thin applicator can be easily introduced inside the body and precisely localized using the most advanced 3D imaging techniques and surgical robots. Minimally invasive hyperthermic applicators (MIHA) have been successfully...

PURPOSE To assess the feasibility of applying trans-applicator intracavitary ultrasonography to image-guided brachytherapy for cervical cancer. MATERIAL AND METHODS For this experiment, a phantom was created and included a polyethylene tube, intended to simulate a tandem applicator, which was inserted into chicken meat and embedded in agar, along with magnetic resonance imaging (MRI)-compatib...

PURPOSE Noninvasive image-guided breast brachytherapy delivers conformal HDR (192)Ir brachytherapy treatments with the breast compressed, and treated in the cranial-caudal and medial-lateral directions. This technique subjects breast tissue to extreme deformations not observed for other disease sites. Given that, commercially-available software for deformable image registration cannot accuratel...

The purpose of this investigation was to examine the effects of beta-adrenergic and muscarinic cholinergic agonists on protein phosphorylation in intact frog atrium. beta-Adrenergic agonists increase and muscarinic agonists decrease 32P incorporation into a 165,000-dalton (165K) protein within less than 1 min. The concentrations of isoproterenol that produce increases in 32P incorporation into ...

Interactions between a murine monoclonal anti-DNA autoantibody (BV17-45) and DNA were examined by direct binding and competitive radioimmunoassays. Binding isotherms constructed by titration of purified BV17-45 with a series of distinct 32P-labeled double-stranded DNA ([32P]dsDNA) fragments were super-impossible, suggesting: 1) BV17-45/[32P]dsDNA binding is independent of dsDNA size using fragm...

Murine immune interferon (Mu-IFN-gamma) can be radiolabeled with [gamma-32P]ATP by the catalytic subunit of cAMP-dependent protein kinase. The resulting 32P-labeled Mu-IFN-gamma (32P-Mu-IFN-gamma) with high radiological specific activity (60-260 muCi/micrograms) retains biological activity. Acid hydrolysis of 32P-Mu-IFN-gamma or 32P-labeled human IFN-gamma leads to the release of [32P]phosphose...

PURPOSE In this study, we present the clinical implementation of a novel transoral balloon centering esophageal applicator (BCEA) and the initial clinical experience in high-dose-rate (HDR) brachytherapy treatment of esophageal cancer, using this applicator. MATERIAL AND METHODS Acceptance testing and commissioning of the BCEA were performed prior to clinical use. Full performance testing was...

Addition of the four deoxyribonucleosides in vitro to 6C3HED mouse ascites tumor stimulates 32P incorporation into DNA and depresses incorporation into RNA at concentrations greater than 10~4 M. The four ribonucleosides stimulate incorporation into both DNA and RNA. Hydroxyurea blocks the ribonucleoside stimulation of 32P incorporation into DNA but not RNA. The inhibition of 32P incorporation i...

Addition of the four deoxyribonucleosides in vitro to 6C3HED mouse ascites tumor stimulates 32P incorporation into DNA and depresses incorporation into RNA at concentrations greater than 10~4 M. The four ribonucleosides stimulate incorporation into both DNA and RNA. Hydroxyurea blocks the ribonucleoside stimulation of 32P incorporation into DNA but not RNA. The inhibition of 32P incorporation i...