The purpose of this study was to examine the potential application of Sorbitol, Sucrose and Gelatin in combination with glycerol in order to Theileria annulata infected cell line cryo-preservation. The efficiency of the freezing methods was compared by assessing the viability and plating efficiency of cells after resuscitation, dilution and equilibration in cell culture medium. As a result, the...

Hypohydration occurs during cold-air exposure (CAE) through combined effects of reduced fluid intake and increased fluid losses. Because hypohydration is associated with reduced physical performance, strategies for maintaining hydration during CAE are important. Glycerol ingestion (GI) can induce hyperhydration in hot and temperate environments, resulting in greater fluid retention compared wit...

The halotolerant green alga Dunaliella salina (isolate SEA003) recently isolated from salt march at Zaranik nature reserve, North Sinai , (Egypt) was cultivated in the arti ficial seawater medium (ASW) with di fferent sal ini ty levels (0.5, 1, 2, 3, and 4 M), under continuous i llumination of two dif ferent li ght intensities: 50 and 200 μmol m 2s , during 30 day of incubation. The maximum gro...

?Crvena Ranka? is one of the oldest autochthonous plum cultivars in Serbia. However, it endangered due to long-term pathogen pressure and continuous suppression by commercially important therefore faced with genetic erosion. This study was carried out investigate suitability two efficient simple cryopreservation methods using aluminium cryo-plates for its conservation. Precultured shoot tips th...

wych elm (ulmus glabra hudson) is one of the forest species in hyrcanian forests of iran and considered as threatened species due to its narrow ecological range, over-exploitation, as well as dutch elm disease. in order to evaluate the possibility of cryopreservation of ulmus glabra seeds for long-term preservation, seeds were collected from natural habitats and three pre-treatment methods incl...

One of the strategies to prevent insulin resistance is to reduce circulating free fatty acids (FFA). The aim of this study is to assess the effect of an oral lactulose load on fatty acid metabolism in overweight subjects. Eight overweight subjects received a primed constant intravenous infusion of [1-(13)C]acetate and of [1,1,2,3,3-(2)H(5)]glycerol for 9 h. After 3 h of tracer infusion, patient...

In an effort to improve industrial production of 1,3-propanediol (1,3-PD), we engineered a novel polycistronic operon under the control of the temperature-sensitive lambda phage P(L)P(R) promoter regulated by the cIts857 repressor and expressed it in Escherichia coli K-12 ER2925. The genes for the production of 1,3-PD in Clostridium butyricum, dhaB1 and dhaB2, which encode the vitamin B(12)-ind...

Elevations in the mass of ether-linked diglycerides (i.e. 1-O-alk-1'-enyl-2-acyl-sn-glycerol (AAG) and 1-O-alkyl-2-acyl-sn-glycerol (Alkyl AG)) during cellular activation are prolonged in comparison to their 1,2-diacyl-sn-glycerol (DAG) counterparts. Since the metabolic removal of DAG is determined, in large part, by the rate of its phosphorylation by diglyceride kinase, we quantified differenc...

BACKGROUND Glycerol is the major by-product accounting for up to 5% of the carbon in Saccharomyces cerevisiae ethanolic fermentation. Decreasing glycerol formation may redirect part of the carbon toward ethanol production. However, abolishment of glycerol formation strongly affects yeast's robustness towards different types of stress occurring in an industrial process. In order to assess whethe...

Palmitate, glucose, and glycerol oxidation to CO(2) have been investigated in the fasted state in ten normal subjects and nine patients (six hyperlipoproteinemias, one xanthomatosis, and two glycogenosis) after intravenous injection of [1-(14)C]palmitate, [1-(14)C]glucose, or [1-(14)C]glycerol in tracer amounts. The specific activities and concentrations of plasma palmitate, glycerol, or glucos...