BACKGROUND Unambiguous identification of nontypeable Haemophilus influenzae (NTHi) is not possible by conventional microbiology. Molecular characterisation of phenotypically defined NTHi isolates suggests that up to 40% are Haemophilus haemolyticus (Hh); however, the genetic similarity of NTHi and Hh limits the power of simple molecular techniques such as PCR for species discrimination. METHO...

Several PCR-based methods are available for walking from a known region to an unknown region in cloned or uncloned genomic DNA. The methods are of three types: inverse PCR (1), randomly primed PCR (2) and adaptor ligation PCR (3-6). However, these methods have not been generally applied to walking in uncloned genomic DNA because they are either complicated or inefficient. Recent improvements to...

Abstract Background The prevention of COVID-19 in vulnerable populations is a global health priority. EVADE was phase 2/3 multicenter, double-blind, randomized, placebo-controlled trial adintrevimab, an extended–half-life monoclonal antibody, for post-exposure (PEP) and pre-exposure prophylaxis (PrEP) symptomatic COVID-19. Methods Eligible participants (vaccine-naïve, aged ≥12 years) were rando...

Objetivos: Avaliar o conhecimento de estudantes ensino médio acerca suporte básico vida (SBV). Método: Estudo realizado em escola pública, com médio. Foram seguidos os passos: 1) aplicação um questionário, objetivo averiguar conhecimentos prévio dos alunos sobre tema; 2) apresentação uma conferência dialogada a cadeia sobrevivência situações parada cardiorrespiratória (PCR); 3) desenvolvimento ...

Anaplasma marginale (A. marginale) is a worldwide pathogen that infects variety ofruminants, but mostly cattle. The present study aimed to describe an isolation technique for A. marginale, using chicken embryo Þ broblast (CEF) cell culture. Blood and tick samples were collected from 5 calves 2 3 months old, which considered be infected with A.marginale due anemia, jaundiced mucous membranes, pr...

Mutations in the gene encoding filaggrin (FLG) have been identified as cause of ichthyosis vulgaris (IV) and shown to be major predisposing factors for atopic dermatitis. The profilaggrin protein consists 10-12 monomer repeats arranged tandem, which arise from an ultra-long sequence exon 3 comprised near-indistinguishable repeats, demonstrating intragenic copy number variations (CNVs). As such,...