On the basis of pIP501, a green fluorescent protein (GFP)-tagged monitoring tool was constructed for quantifying plasmid mobilization among Gram-positive bacteria and between Gram-positive Enterococcus faecalis and Gram-negative Escherichia coli. Furthermore, retromobilization of the GFP-tagged monitoring tool was shown from E. faecalis OG1X into the clinical isolate E. faecalis T9.

BACKGROUND Respiratory infections with sputum production are a major reason for physician visits, diagnostic testing, and antibiotic prescription in the United States. We sought to determine whether the simple characteristic of sputum color provides information that impacts resource utilization such as laboratory testing and prescription of antibiotics. METHODS Out-patient sputum samples subm...

This study examines the effects of tillage, residue management and cropping system intensification through the inclusion of green gram on the performance of the rice-wheat (RW) system in NW India. We hypothesized that zero tillage (ZT) with residue retention provides a means of sustainably intensifying the RW system through lower production costs and higher economic profitability, whilst at the...

Rice flour and green gram flour (sprouted as well as unsprouted) were blended in different proportions with apple pulp, sugar, milk and water for formulation of 12 hypoallergic diets. The formulations were studied for physico-chemical properties, antinutrient content (phytate and oxalate) and in vitro protein digestibility. Blending and sprouting significantly affected all the studied parameter...

A field experiment was conducted at Eastern Block Farm, Department of Agronomy, TNAU, Coimbatore, during the summer 2019 with objective finding outthe nutrient uptakeandthe postharvest soil statusof Extra Long Staple (ELS) cotton under methods crop establishment, intercropping and management. The laid out in a randomized block design three replications. treatments consisted,viz.T1: Transplantin...

Roselle (Hibiscus sabdariffa) calyx was fermented traditionally and by controlled method with pure strains of Aspergillus flavus and Saccharomyces cerevisiae isolated from the fermentation. The proximate composition revealed that the sample fermented traditionally had the highest protein content of 14.7±2.5%. There was a significant decrease (Pd≤0.05) in the antinutrient content (phytate and ta...

A study was conducted to determine the effects of packaging materials, seasonality, storage temperature and time on physiochemical and antioxidant properties of roselle-mango juice blends. Roselle extract (20%, 40%, 60%, and 80%) was mixed with mango juice and stored in glass and plastic bottles at 4°C and 28°C. Total soluble solids, pH, titratable acidity, reducing sugar, color, vitamin C, tot...

In this study effect of irrigation managements including irrigation after 100 and 200 mm pan evaporation as normal and deficit irrigation respectively was investigated in Roselle plants. Effects of humic acid (including 0 and 4 kg ha-1) and mycorrhizal inoculants (including Glomus versiforme (GV) and Rhizophagus irregularis (RI)) were also studied on growth, yield and mycorrhizal symbiosis inde...

We show that a pH-sensitive derivative of the green fluorescent protein, designated ratiometric GFP, can be used to measure intracellular pH (pHi) in both gram-positive and gram-negative bacterial cells. In cells expressing ratiometric GFP, the excitation ratio (fluorescence intensity at 410 and 430 nm) is correlated to the pHi, allowing fast and noninvasive determination of pHi that is ideally...