نتایج جستجو برای: 248 polymorphic bands were amplified primers opa

تعداد نتایج: 3561191  

Journal: :علوم گیاهان زراعی ایران 0
مرضیه نوری گوغری دانشجوی کارشناسی ارشد دانشکدۀ کشاورزی دانشگاه ولی عصر رفسنجان حسین دشتی دانشیار دانشکدۀ کشاورزی دانشگاه ولی عصر رفسنجان شهاب مداح حسینی استادیار دانشکدۀ کشاورزی دانشگاه ولی عصر رفسنجان الهام دهقان مربی پژوهشی دانشکدۀ کشاورزی دانشگاه ولی عصر رفسنجان

genetic diversity is the basis success of improving the quality and quantity of crops. recognizing of genetic diversity and genetic potential of each plant species in plant breeding is necessary. in this study genetic diversity of 30 lentil samples belonging to collection of the college of agriculture of krage were evaluated using rapd molecular markers. rapd products were scored for presence (...

2011
A. M. Mohamed A. T. Sharaf El-Din A. H. Mohamed M. R. Habib

The first generation of Biomphalaria snails collected from five Egyptian governorates (Giza, Fayoum, Kafr El-Sheikh, Ismailia and Damietta) were subjected to species-specific PCR assays and the results showed that snails collected from the field were B. alexandrina, and there was no evidence for the presence of B. glabrata. The snails were subjected also to RAPD-PCR technique. The results showe...

Journal: :Forensic science international 2007
Lin He Sibao Wang Xuexia Miao Hong Wu Yongping Huang

Necrophagous fly is the most common insect evidence collected during a death investigation. We analyzed the DNA polymorphism among the five forensically important fly species, namely, Lucilia sericata, Aldrichina grahami, Chrysomya megacephala, Parasarcophaga crassipalpis and Musca domestica using inter simple sequence repeat (ISSR) method. Nine ISSR primers selected from 18 primers could ampli...

2003
Chengxin Fu Yingxiong Qiu

Random Amplified Polymorphic DNA (RAPD) markers were used to measure genetic diversity of Changium smyrnioides Wolff (Apiaceae), a genus endemic to eastern China and an endangered medicinal plant, collected from five populations along the Yangzi River. A total of 92 amplified bands were scored from the 13 RAPD primers, and a mean of 7.1 amplified bands per primer and 69% (64 bands) percentages ...

Journal: :علوم باغبانی ایران 0
شهلا کیان امیری دانشجوی سابق کارشناسی ارشد پردیس کشاورزی و منابع طبیعی دانشگاه تهران محمد اسماعیل حسنی استادیار پردیس کشاورزی و منابع طبیعی دانشگاه تهران ذبیح اله زمانی استاد پردیس کشاورزی و منابع طبیعی دانشگاه تهران

genetic diversity of 36 genotypes of dwarf and semi-dwarf apple rootstocks along with seven iranian dwarf rootstocks were investigated by use of rapd dna markers. a set of 100 random primers was utilized in pcr reaction from which 10 primers resulted in good amplification with high polymorphism. among primers, 10 selected rapd primers produced 160 bands, in which, 136 were polymorphic. cluster ...

Journal: :Journal of Biology, Agriculture and Healthcare 2023

Cucurbits are economic crops in Nigeria, which not only taken to overcome nutritional deficiency but also good sources of income for farmers. The taxonomy the members family Cucurbitaceae had been a challenge botanists Nigeria as result many overlaps group, hence confusion proper identification members. Randomized Amplified Polymorphic DNA (RAPD) markers were employed study genetic diversity am...

Journal: :Genetics and molecular research : GMR 2010
K Demir M Bakir G Sarikamiş S Acunalp

Eggplant is a major crop in Turkey, which produces more of this crop than all of Europe; consequently, germplasm resources are of concern for the country. Molecular characterization of eggplant genotypes collected from different geographical regions of Turkey was carried out using SSR and RAPD markers. With amplification of five SSR loci, the number of alleles per microsatellite locus ranged fr...

Journal: :Canadian journal of microbiology 2002
Fajun Chen Paul H Goodwin Adalat Khan Tom Hsiang

Eighty-seven isolates of Colletotrichum graminicola, mostly from Agrostis palustris, were collected in grass fields, most of which were in Ontario, Canada. Specific primers were designed to amplify the mating-type (MAT) genes and, among 35 isolates tested, all yielded a band of the expected size for MAT2. For six isolates, the MAT2 PCR products were sequenced and found to be similar to that rep...

S.A. Talebzadeh S.M. Hesamzade Hejazi, T. Valinassab

ISSR markers were used to determine the genetic variation and sex determination of the three life-stages of 41 samples of Gracilaria corticata from two regions of the Persian Gulf and Oman Sea namely Bostaneh and Lipar. The specimens were cultured in PES media to observe the different life stages. DNA was extracted by modified CTAB method. After screening of 20 ISSR primers, four primers were s...

Journal: :Genetics and molecular research : GMR 2010
D Braganholi M Bélo B W Bertoni A L Fachin R O Beleboni S M Zingaretti

Random amplified polymorphic DNA (RAPD) was used to detect polymorphisms among Zaprionus indianus fly populations collected from six municipalities in the States of São Paulo and Minas Gerais, Brazil. This species is an important, recently introduced fruit fly pest of figs and other fruit. Among 21 primers, 16 produced 73 reproducible polymorphic fragments; primer AM-9 produced the greatest num...

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