Sections of hypocotyls, roots and leaves from Pentalinon andrieuxii plantlets were transiently transformed with Agrobacterium tumefaciens LBA4404 bearing the binary plasmid pCAMBIA2301 with an interrupted β-glucuronidase (GUS) gene. Histochemical GUS assays showed transient gene expression in all infected tissues, being older roots those which displayed the most intense GUS staining. To our kno...

T h e infectivity of second-stage larvae of Meloidogyne spp. is a function of soil temperature, aeration, moisture, texture, and density, as well as a funct ion of the distance the second-stage larvae have to travel pr ior to root penetra t ion (3, 4). Because eggs may require up to 2 weeks to hatch under greenhouse conditions, infection periods with larvae are normal ly shorter than those with...

Two experiments were conducted to assess nutrient partitioning in coffee (Coffea arabica cv. Typica land race Guatemala) infected with Meloidogyne konaensis. Nutrient levels were quantified from soil, roots, and leaves. In the first experiment, 500-cm3 aliquants of a Kealakekua Andisol were infested with four initial population densities of M. konaensis ranging from 0 to 1,500 freshly hatched s...

In mycorrhizal symbioses, susceptibility of a host plant to infection by fungi is influenced by environmental factors, especially the availability of soil phosphorus. This study describes morphological and biochemical details of interactions between a vesicular-arbuscular mycorrhizal (VAM) fungus and potato (Solanum tuberosum L. cv Russet Burbank) plants, with a particular focus on the physiolo...

The rhizosphere of Trifolium repens and Lolium perenne was divided into three fractions: the bulk soil; the soil adhering to the roots; and the washed roots (rhizoplane and endorhizosphere). After isolation and puri®cation of DNA from these fractions, 16S rDNA was ampli®ed by PCR and cloned to obtain a collection of 16S rRNA genes representative of the bacterial communities of these three fract...

Roots of Angelica sinensis (Danggui) have been used in promoting blood circulation as herbal medicine for over 2000 years in China. Another species of Angelica roots called A. gigas is being used in Korea. To reveal the efficiency of different Angelica roots, the chemical and biological properties of Angelica roots from different cultivated regions were compared. Roots of A. sinensis contained ...

AIM The aim of this study was to determine the root and canal morphology of maxillary first premolars collected from an indigenous Saudi population. METHODS AND MATERIALS A total of 246 extracted teeth were collected and washed immediately after extraction then stored in 10% formalin solution. The teeth were debrided then dehydrated with ascending concentrations of alcohol and cleared by imme...

Excised tomato roots infected with Meloidogyne javanica produced ethylene at 3-6 times the rate of noninfected roots. This increase in ethylene production started 5 days after inoculation. Gall growth and ethylene production in infected roots were accelerated by 1-aminocyclopropane-1-carboxylic acid (ACC), indole acetic acid (IAA), and ethrel known as ethylene production stimulators. When inhib...

abstract in this study the effect of combination of trichoderma harzianum bi and salicylic acid were studied on root knot nematode (meloidogyne javanica) disease on tomato under green house conditions. the roots of tomato seedlings at six-leaf stage were inoculated with suspensions of fungal antagonist (106 conidia/ ml), 5mm salicylic acid and 2000 second-stage juvenile (j2). combined applicati...

Normal and Plasmodium berghei (NYU-2 strain)-infected murine erythrocytes display substantially different patterns of plasma membrane phosphoproteins phosphorylation. Intact erythrocytes (normal and parasite infected) incubated with 32Pi and isolated washed erythrocyte plasma membranes incubated with gamma-32P-ATP were analyzed for phosphoproteins by SDS PAGE and autoradiography. Two new phosph...