Large body surface area (BSA) could be a pitfall in long-term peritoneal dialysis. We analyzed the viability of the peritoneum in terms of adequacy and technique survival in patients of varying BSA. We grouped our PD patients into three categories (BSA < or = 1.59 m2, BSA 1.60 - 1.79 m2, and BSA > or = 1.80 m2) and used the Student t-test to compare the mean weekly Kt/V urea between the groups ...

BACKGROUND Although many studies have investigated macromolecular uptake in the stomach and small intestine, little is known about macromolecular uptake in the colon. AIMS To investigate the mechanisms involved in the transport of large antigenically intact macromolecules across the proximal and distal colonic epithelium in the rabbit. METHODS The mucosal to serosal movement of bovine serum...

Determination of the BSA-palmitate high-affinity binding constant ( K a) traditionally relied on the heptane-water partitioning technique. We used this technique to calculate K a for the BSA-[3H]palmitate complex, to determine if K a was independent of protein concentration, and to determine if the unbound [3H]palmitate concentration is constant at different BSA concentrations using constant BS...

PURPOSE Advanced glycation end products of BSA (AGE-BSA) participate in the pathogenesis of diabetic vascular disease. However, the role of AGE-BSA in diabetic retinopathy, especially in retinal neovascularization, remains incomplete. This study aimed to determine the contributions of AGE-BSA in the endothelial-to-mesenchymal transition (EnMT) of cultured human and monkey endothelial cell lines...

Protein sedimentation sans cryoprotection is a new approach to magic angle spinning (MAS) and dynamic nuclear polarization (DNP) nuclear magnetic resonance (NMR) spectroscopy of proteins. It increases the sensitivity of the experiments by a factor of ∼4.5 in comparison to the conventional DNP sample preparation and circumvents intense background signals from the cryoprotectant. In this paper, w...

Anti 5-methyl-cytidine antibodies might be useful agents for the detection and localization of 5-methyl-cytidine of nucleic acids, but only if the antibodies recognize this nucleoside with sufficient specificity. A conjugate containing 18 moles of 5-methyl-cytidine per mole of BSA was prepared and antibodies directed against this nucleoside hapten were produced by immunization of rabbits (as de...

PURPOSE High-mobility group box 1 protein (HMGB1) has been reported to be a potent proangiogenic factor induced by inflammatory stress. In this study, we explore the role of HMGB1 in advanced glycation end products (AGEs)-induced vascular endothelial growth factor A (VEGF-A) production in rat retinal ganglion cell line 5 (RGC-5) cells. METHODS The VEGF-A protein and mRNA levels in conditioned...

An indirect immunoassay for detecting antigen was developed. It was based on fluorescence resonance energy transfer (FRET) and quenching of gold nanoparticles. Bovine serum albumin (BSA) was chosen as model antigen. Fluorescein isothiocyanate (FITC) was attached to anti-BSA antibody (anti-BSA–FITC) as FRET donor, while BSA was conjugated to gold nanoparticles (GNPs–BSA) as FRET acceptor. The fo...

The effect of the presence sodium dodecyl sulfate (SDS) on denaturation bovine serum albumin (BSA) has been studied using 36 m small-angle neutron scattering (SANS) BATAN spectrometer (SMARTer). data reduction used Graphical Reduction and Analysis SANS Program (GRASP) software, fitting process IGOR software. was identified by observing changes BSA globular structure. experimental results showed...