Severe acidic pH-activated chloride channel (ICl,acid) has been found in various mammalian cells. In the present study, we investigate whether this channel participates in reactions of the thoracic aorta to severe acidosis and whether it plays a role in hypertension. We measured isometric contraction in thoracic aorta rings from spontaneously hypertensive rats (SHRs) and normotensive Wistar rat...

Pancreatic cancer cell lineswithmutated ras underwent an alternative formof cell death (aponecrosis) when treated concomitantly with clinically achievable concentrations of arsenic trioxide, ascorbic acid, and disulfiram (Antabuse; AAA). AAA’smajor effects aremediated through generation of intracellular reactive oxygen species (ROS) and more than 50% decline in intracellular ATP. N-acetyl cyste...

We have previously identified the suramin analog 4,4',4'',4'''-(carbonylbis(imino-5,1,3-benzenetriylbis(carbonylimino)))tetrakis-benzene-1,3-disulfonic acid (NF449) as a low nanomolar potency antagonist of recombinant P2X(1) receptors. Here, we characterize, by two-electrode voltage-clamp electrophysiology, three isomeric suramin analogs designated para-4,4',4'',4''''-(carbonylbis(imino-5,1,3-b...

A simple and sensitive kinetic-spectrophotometric method is described for the determination of vanadium and the possible mechanism of catalytic reaction is proposed. The method is based on the vanadium(V)-catalyzed oxidation of 1,8-diaminonaphthalene (DNA) by potassium bromate (Tiron as activator) at pH 3.8 and 40 degrees C. The reaction was monitored spectrophotometrically by measuring the inc...

The decomposition of sulfopropyl sulfonate ~SPS, 4,5-dithiaoctane-1,8-disulfonic acid! in acid copper electroplating chemistries was studied using scanning electrochemical microscopy ~SECM!, UV-visible spectroscopy, high pressure liquid chromatography, liquid chromatography mass spectrometry, and electron spin resonance. The primary decomposition product is proposed to be the thiolsulfonate of ...

The extracellular pH (pi I,.) in many solid tumors is often lower than in normal tissues. Cells may survive conditions of acid pi 1, because antiports in their membrane exchange Na* for 11+,or 11CX)., for Cl~, and thus regulate the intracellular pH (pH,). We have therefore assessed the effects of drugs which interfere with regulation of pi I; on survival of Chinese hamster ovary and human bladd...

The extracellular pH (pHe) in many solid tumors is often lower than in normal tissues. Cells may survive conditions of acid pHe because antiports in their membrane exchange Na+ for H+, or HCO3- for Cl-, and thus regulate the intracellular pH (pHi). We have therefore assessed the effects of drugs which interfere with regulation of pHi on survival of Chinese hamster ovary and human bladder cancer...