In this study, two new freshwater fish species, Gambusia holbrooki Girard, 1859 and Petroleuciscus borysthenicus (Kessler, 1859) were reported from Simenlik-Akgöl Lagoon in Yeşilırmak Basin (Samsun). Turkey has a rich biodiversity terms of fish. Because climate change, periodic ichthyofauna monitoring updating fauna are critical. holbrooki, Eastern mosquitofish, is an invasive species widesprea...