In the present investigation 28 genotypes of soybean were analysed using 37 start codon targeted (SCoT) markers and primers produced 260 DNA fragments with an average 7.03 bands per primer. From these primers, SCoT 33 65 wos most polymorphic, where 10 polymorphic amplification products detected. The lowest number amplified (2) was detected by 8, 14, 19, 31 59. Of bands, 200 (77.27 %) 5.41 To de...

the genetic diversity of 48 wheat genotypes was studied by rapd markers at 2012 in laboratory of department of agronomy and plant breeding, university of mohaghegh ardebili. 10 primers from 70 used primers showed suitable banding patterns. these primers totally produced 73 polymorphic bands with mean 7.3 for each primer. the number of bands for 55 and 59 primers were 5 and 11, respectively. the...

Three different DNA-based techniques, Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeat (ISSR) and Amplified Fragment Length Polymorphism (AFLP) markers, were used for fingerprinting Dactylis glomerata genotypes and for detecting genetic variation between the three different subspecies. In this study, RAPD assays produced 97 bands, of which 40 were polymorphic (41.2%). The I...

A repeat sequence 682 base pairs (bp) long produced by cleavage of human DNA with Xba I restriction enzyme is composed of four tandemly arranged subunits with lengths of 171, 170, 171, and 170 bp each. The sequence organization of the 682 bp Xba I repeat bears a striking resemblance to other complex satellite DNAs of primates, including the Eco RI human alpha satellite family which also occurs ...

the first step in breeding programs is detecting the genetic diversity in breeding materials. molecular markers help to reduce costs and time in breeding programs. genetic variation in 49 genotypes of tobacco were studied using 12 issr markers, 3 retrotransposon markers and the combination of issr and retrotransposons. one hundred forty seven polymorphic bands were produced using 16 primers. to...

Random amplified polymorphic DNA (RAPD) PCR is a feasible method to evaluate genotoxin-induced DNA damage and mutations. In this study, Lactobacillus plantarum ATCC 14917T, Enterococcus faecium DSMZ 20477T, Escherichia coli PQ37 and Saccharomyces cerevisiae S441 were screened for DNA genetic alterations by DNA fingerprinting using M13 and LA1 primers after treatment with three compounds forming...

two different methods of bayesian segmentation algorithm were used with different band combinations. sequential maximum a posteriori (smap) is a bayesian image segmentation algorithm which unlike the traditional maximum likelihood (ml) classification attempts to improve accuracy by taking contextual information into account, rather than classifying pixels separately. landsat 7 etm+ data with pa...

Genetically, every individual is unique; this may stem from inheritance, geographical locations, and/or environmental interactions. This study examined the possibility of developing a cheap and easy-to-use marker that can distinguish among the three ethnic groups in Nigeria using RAPD-PCR. Five RAPD primers, OPA1-3 and OPC1-2, were randomly selected and used to amplify DNA samples isolated from...