The viral disease classical swine fever (CSF), caused by a Pestivirus, is one of the major causes of economic losses for pig farming. The aim of this work was to validate a RT-qPCR using Taqman for detection of CSF in swine tissues. The parameters for the validation followed the specifications of the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals of the World Organization for A...

GenPlex (Applied Biosystems) is a new SNP genotyping system based on an initial PCR amplification followed by an oligo ligation assay (OLA). The OLA consists of the hybridization of allele and locus specific oligonucleotides (ASOs and LSOs) to PCR products and posterior ligation of ASOs and LSOs. The ligation products are immobilized to microtitre plates and reporter oligonucleotides (ZipChute ...

Among the general requirements to accredit a radon measuring service, outlined in the international standards as EN ISO/IEC 17025, the technical requirements play the main role in the quality assurance of a testing service. The reliability of the assessment of the radon air concentration in workplaces and dwelling is determined by many factors the most important being the physical measuring met...

Adequate glazing (6-10%) of fish fillets prior to frozen storage protects the final product from dehydration, oxidation and quality loss. Excessive glazing (>12%) on the other hand may significantly affect the economic value and end user satisfaction of frozen fish fillets. This paper describes the optimization, validation and application of a gravimetric procedure for the quantification of the...

The applications of ultrasonic Lamb waves are becoming more and frequent diverse in industrial fields. These used non-destructive testing (NDT), structural health monitoring (SHM), for the assessment quality materials, etc. However, due to unusual properties these waves, new or adapted signal processing methods necessary use analysis such wave signals. Based on requirements ISO 17025, work pres...

Abstract This paper presents the preparation, characterisation and validation of two quality control (QC) samples for measurement plutonium uranium contents by isotope dilution mass spectrometry (IDMS). These QC were then used at JRC-Geel G.2′s laboratory to monitor performance IDMS results in line with ISO 17025 17034. The prepared from certified reference materials, namely NBL-126 (Pu) CRM 11...

− One of priorities in research undertaken to support Food Composition Analysis was the production of a range of reference materials relevant to determine nutrients in selected foods. This paper describes the implementation and outcomes of activities on reference material production program and their contribution to international developments in the harmonization of Quality System of Food Compo...

Fisheries are important in Sri Lanka as a food sector as well as an export sector. The main export fish species is yellowfin tuna and main markets are Japan, EU countries and the USA. Yellowfin tuna and swordfish are high in the aquatic food web, therefore accumulation of trace metals can occur. The export goods quality and safety should comply with the WTO, national, regional and international...