Using the PCR, we amplified the 16S ribosomal DNAs (rDNAs) of an Asian strain and an African strain of the uncultured, gram-negative, walled, phloem-limited bacterium-like organism (BLO) associated with citrus greening disease. We evaded coamplification of chloroplast 16S rDNA by using restriction enzymes; the chloroplast 16S rDNA was sensitive to BclI digestion and resistant to EcoRI digestion...

In the present study, out of 264 phosphate (P) solubilizing Bacillus strains isolated from apple rhizosphere, only twelve isolates were found to be efficient (showed most of the plant growth promoting activity) which were further characterized at molecular level using 16S rDNA partial gene sequencing. Out of 12 isolates, MZPSB 207 was found to be most efficient P-solubilizing (864.71 μg/ml) iso...

objective(s) rapid tests for detection of streptococcus agalactiae or group b streptococci (gbs) at the onset of labor are needed to permit early intrapartum antibiotic prophylaxis. this study aimed to evaluate the pcr assays targeting the 16s ribosomal rna gene (16s rdna) for detection of the gbs in comparison with a specific culture method. materials and methods two swabs were used to obtain ...

Background: Identification of the dominant lactic acid bacteria involved in the production of traditional cheese in Semnan could be the initiative to protect national genetic resources and produce industrial cheese with desirable texture and organoleptic characteristics similar to traditional cheeses. The present study aimed to determine the biochemical, physiological, and phenotypic properties...

Studies were conducted on the improvement of A. culicicola identification. This species is phenotypically very similar to A. veronii biotype sobria, A. sobria, and A. allosaccharophila. The sequences of 16S rDNA of A. culicicola isolates show the highest similarity with A. jandaei, A. veronii, and A. caviae. Digestion of 16S rDNA PCR product with AluI and MboI restriction endonucleases allowed ...

In prokaryotes, the ribosomal gene locus contains three rRNA species: 16S, 23S and 5S. The potential of sequencing 16S rDNA of bacteria and archaea for establishing phylogenetic relationships and for use in molecular diagnostics is well-documented. Currently, over 3000 16S rRNA sequences are available in the databases. However, the potential of the 23S rRNA gene as a diagnostic tool has not bee...

l-asparaginase is a candidate enzyme for anti-neoplastic agent againstacute lymphoblasticleukemia and also extensively use in the food industry for prevention of acrylamide formation. l-asparaginase is widely distributed among microorganisms. in this study, marine bacteria were isolated from persian gulf and screened for l-asparaginase activity. production of l-asparaginase was carried out by u...