The development and validation of a microsphere immunoassay (MIA) to detect equine antibodies to the major structural proteins of equine arteritis virus (EAV) are described. The assay development process was based on the cloning and expression of genes for full-length individual major structural proteins (GP5 amino acids 1 to 255 [GP5(1-255)], M(1-162), and N(1-110)), as well as partial sequenc...

Equine piroplasmosis is a tick-borne disease caused by intra-erythrocyte protozoa, Theileria equi and Babesia caballi. The present study aimed to detect piroplasm infection in horses in Kurdestan Province, Iran, through molecular and microscopic approaches.n this study, 186 blood samples were randomly collected from horses of five regions of Kurdestan Province. The Tbs-S/Tbs-A primer set was us...

Adult articular cartilage (AC) has a well described multizonal collagen structure. Knowledge of foetal AC organisation and development may provide a prototype for cartilage repair strategies, and improve understanding of structural changes in developmental diseases such as osteochondrosis (OC). The objective of this study was to describe normal development of the spatial architecture of the col...

Although often considered a strict human pathogen, Streptococcus pneumoniae has been reported to infect and cause pneumonia in horses, although the pathology appears restricted compared to that of human infections. Here we report on the molecular characterization of a group of S. pneumoniae isolates obtained from horses in England and Ireland. Despite being obtained from geographically distinct...

background: brucellosis is a febrile zoonotic infectionand has worldwide distribution among humans as well asanimals. although the seroprevalence of brucellosis in variousanimals has been described in iran, there is only one report onequine brucellosis in the region. objectives: this study wascarried out to determine the seroprevalence of brucellosis inracing clubs and private horse owners in t...

vitamin d deficiency is one of the most preventable challenges worldwide. the aim of this study was to determine the prevalence of vitamin d deficiency among female nurses working at children’s medical center hospital in tehran, iran, due to the risk factor of being a notably long period indoors and the fact that their health status may have consequences on the process of patients’ treatment. a...

We have used dioxygen, not artificial oxidants such as peracids, iodosylarenes, and hydroperoxides, in the generation of a mononuclear nonheme oxoiron(IV) complex, [Fe(IV)(TMC)(O)]2+ (TMC = 1,4,8,11-tetramethyl-1,4,8,11-tetraazacyclotetradecane), from its corresponding Fe(II) complex, [Fe(TMC)(CF3SO3)2]. The formation of oxoiron(IV) species by activating dioxygen was markedly dependent on iron(...

Neutron detection efficiency of superheated drops of Freon-22 for neutrons obtained from a 3 curie Am-Be neutron source has been reported in this paper. Although Freon-22 having lower boiling point than many other similar liquids (e.g.Freon-12, Freon-114, Isobutane) is expected to be more sensitive to neutrons ,it has not been reported so far and therefore this paper constitutes the first repor...