When HeLa cell nuclei were treated with micrococcal nuclease (nucleate 3-oligonucleotidohydrolase, EC 3.1.4.7), lysed, and centrifuged, the supernatant from early digests contained two predominant classes of polynucleosomes of repeat size 8N and 16N. With increasing digestion time, the 16 N polynucleosome appeared to be cleaved to the 8N species and finally to the basic subunit of chromatin. Th...

DNA polymerase slippage occurs frequently in tracts of a tandemly repeated nucleotide, and such slippage events can be genetically detected as frameshift mutations. In long mononucleotide runs, most frameshift intermediates are repaired by the postreplicative mismatch repair (MMR) machinery, rather than by the exonucleolytic proofreading activity of DNA polymerase. Although mononucleotide runs ...

We consider the problem of periodic graph exploration by a finite automaton in which an automaton with a constant number of states has to explore all unknown anonymous graphs of arbitrary size and arbitrary maximum degree. In anonymous graphs, nodes are not labeled but edges are labeled in a local manner (called local orientation) so that the automaton is able to distinguish them. Precisely, th...

Planktonic foraminifera from a continuous Oligocene succession with clear magnetochronology and sediment cycles at Ocean Drilling Program Site 1218 (equatorial Pacific Ocean) were studied in the interval from 27 to 30 Ma. Paragloborotalia taxa are common and we examined their size, relative abundance, and stable isotopes. Multispecies stable isotope data indicate the depth habitats of Oligocene...

We consider networks of processes which interact with beeps. In the basic model defined by Cornejo and Kuhn [7], which we refer to as the BL variant, processes can choose in each round either to beep or to listen. Those who beep are unable to detect simultaneous beeps. Those who listen can only distinguish between silence and the presence of at least one beep. Beeping models are weak in essence...

The implication of the Bcg locus in the control of natural resistance to infection with a live vaccine strain (LVS) of the intracellular pathogen Francisella tularensis was studied. Analysis of phenotypic expression of natural resistance and susceptibility was performed using mouse strains congenic at the Bcg locus. Comparison of the kinetics of bacterial colonization of spleen showed that B10....

In this paper we consider Maker-Breaker games, played on the edges of sparse graphs. For a given graph property P we seek a graph (board of the game) with the smallest number of edges on which Maker can build a subgraph that satisfies P. In this paper we focus on global properties. We prove the following results: 1) for the positive minimum degree game, there is a winning board with n vertices ...

BACKGROUND Carcinoma of the prostate is one of the common tumours of old age in men. This cross sectional study was conducted to detect carcinoma of prostate in clinically benign enlarged gland and to evaluate the efficacy of Digital rectal Examination in detection of prostatic cancer in patients presented at Khyber Teaching Hospital, Peshawar from July 1998 to July 1999. METHODS Patients pre...

BACKGROUND AND AIMS In order to fabricate prostheses with high accuracy and durability, soldering techniques have been introduced to clinical dentistry. However, these prostheses always fail at their solder joints. The purpose of this study was to evaluate the effect of gap distance on the tensile strength of base metal solder joints. MATERIALS AND METHODS Based on ADA/ISO 9693 specifications...

We have developed a chemical-enzymatic synthetic approach to gonadotropin releasing hormone G lp '-H is2-T r p 3-S er4-T y r5-G ly 6-L e u 7-A r g 8-P r o 9G ly 10N H 2 (GnRH) which enables one to perform the synthesis efficiently according to minimal protection strategy with all reactive side chains o f amino acids unprotected. Five peptide bonds His2-T r p 3, Tyr5-G ly 6, Leu7-A r g 8 and Trp...