Impact assessment of contaminants in soil is an important issue in environmental quality study and remediation of contaminated land. A random amplified polymorphic DNA (RAPD) 'fingerprinting' technique was exhibited to detect genotoxin-induced DNA damage of plants from heavy metal contaminated soil. This study compared the effects occurring at molecular and population levels in barley seedlings...

Jacaranda decurrens (Bignoniaceae) is an endemic species of the Cerrado with validated antitumoral activity. The genetic diversity of six populations of J. decurrens located in the State of São Paulo was determined in this study by using molecular markers for randomly amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP). Following optimization of the amplification ...

mahaleb (prunus mahalab l.) is used as a rootstock for cherries in iran and many other countries. in this research, rapd markers were used to determine the genetic diversity among eight genotypes of mahaleb evaluated in two separate research grardens in karaj, iran. these genotypes are used as mother plants for seed production. totally 40 random primers were examined for pcr reactions on the te...

We optimized RAPD techniques by increasing the length of RAPD primers and performing a strict screening of PCR annealing temperature to distinguish 60 sweet orange cultivars from the Research Institute of Pomology at the Chinese Academy of Agricultural Sciences. A new approach called cultivar identification diagram (CID) was used to improve the efficiency of RAPD markers for cultivar ident...

The study focuses on the analysis of population genetic structure of Culex quinquefasciatus from various larval habitats using RAPDPCR. The population differentiation parameters, genetic distances, molecular variance and cluster analysis were done using POPGENE, GenALEx and MEGA softwares. Out of 140 RAPD primers screened, four primers exhibited distinct RAPD banding patterns showing up to 100%...

Three different DNA-based techniques, Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeat (ISSR) and Amplified Fragment Length Polymorphism (AFLP) markers, were used for fingerprinting Dactylis glomerata genotypes and for detecting genetic variation between the three different subspecies. In this study, RAPD assays produced 97 bands, of which 40 were polymorphic (41.2%). The I...

Rice is a cereal crop that belongs to the graminae family of high economic value, and main food for more than portion world's population. This study aims determine relationship genetic rice in Kampar by RAPD marker. Eight genotypes from district were amplified using eight primers (OPA 5, OPB 7, OPC 19, OPD 2, 3, 8, 11, 13). The analysis molecular data was performed NTSys pc -2.02 GenAlex 6.1 . ...

rapd markers were employed to assess the genetic diversity of 34 iranian wild edible asparagus (a. officinalis) genotypes as well as cv. mary washington and a. persicus. in total, 80 rapd primers were employed for pcr reactions, among which, 18 revealed reasonable polymorphism. the 18 primers produced 175 bands, of which, 160 were polymorphic (91.4% polymorphism). cluster analysis of the genoty...

RAPD-PCR method was used for systematic study and revealing the genetic relationships in Malus by using 7 apple cultivars from some mid Iraqi regions, DNA of fresh leaves extracted modified protocol CTAB, 22 prechosen random decamer primers were applied to detect genotypes. four gave reproducible appeared polymorphism RAPD profile, a total 48 bands produced out which 36 polymorphic, results ari...

The variation in two Bruguiera species in populations from different climatic and macrogeographic regions along the western to southeastern coastal area in Sri Lanka was studied using RAPD (Random Amplified Polymorphic DNA). Intraand interspecific variation was observed using 20 primers of the 45 primers screened. Some primers showed diagnostic banding patterns and allowed unambiguous different...