Objective(s):To culture thein vitro mouse embryonic stem cells (mESCs) and to direct their  differentiation to germ-line cells; in present study we used a vector backbone containing the fusion construct Stra8-EGFP to select differentiated ES cells that entered meiosis.  Retinoic acid was used to differentiate embryonic stem cells to germ cells. Materials and Methods: A fragment of Stra8 gene pr...

Knowing the nucleotide sequence of the cholera toxin operon, we designed oligonucleotide primers for its-PCR amplification from local clinical isolates of V. cholerae. The resulting amplification product was cloned in a common pUC18 vector. Subsequently, a part of this operon encoding the cholera toxin Bsubunit (CTB) was reamplified and cloned between the BamH1 and EcoR1 sites of the same ...

In this work we deal with actions of vector groupoid which is a new concept in the literature‎. ‎After we give the definition of the action of a vector groupoid on a vector space‎, ‎we obtain some results related to actions of vector groupoids‎. ‎We also apply some characterizations of the category and groupoid theory to vector groupoids‎. ‎As the second part of the work‎, ‎we define the notion...

Objective(s): Diabetes mellitus (DM) is a major worldwide public health challenge, for which gene therapy offers a potential therapeutic approach. To date, no systematic review or meta-analysis has been published in this area, so we examined all relevant published studies on rodents to elucidate the overall effects of gene therapy on bodyweight, intraperitoneal glucose...

Let $M$ be an orientable hypersurface in the Euclidean space $R^{2n}$ with induced metric $g$ and $TM$ be its tangent bundle. It is known that the tangent bundle $TM$ has induced metric $overline{g}$ as submanifold of the Euclidean space $R^{4n}$ which is not a natural metric in the sense that the submersion $pi :(TM,overline{g})rightarrow (M,g)$ is not the Riemannian submersion. In this paper...

Blinatumomab, the bispecific T cell engager, has been demonstrated as the most successful BsAb to date. Throughout the past decade, vector design has great importance for the expression of monoclonal antibody in Chinese hamster ovary (CHO) cells. It has been indicated that expression plasmids based on the elongation factor-1 alpha (EF-1 alpha) gene and DHFR selection marker can be highly effect...

a‎s we know, developing mathematical models and numerical procedures that would appropriately treat and solve systems of linear equations where some of the system's parameters are proposed as fuzzy numbers is very important in fuzzy set theory. for this reason, many researchers have used various numerical methods to solve fuzzy linear systems. in this paper, we define the concepts of midpoint a...

this paper is concerned with the development of a novel classifier for automatic mass detection of mammograms, based on contourlet feature extraction in conjunction with statistical and fuzzy classifiers. in this method, mammograms are segmented into regions of interest (roi) in order to extract features including geometrical and contourlet coefficients. the extracted features benefit from...

background: development of an effective vaccine is highly needed in order to restrict the aids pandemic. dna vaccines initiate both arms of immunity without the potential of causing disease. hiv-1 p24 and gp41 (gag and env) proteins play important roles in viral pathogenesis and are effective candidates for immune induction and vaccine design. objective: in this study, new dna vaccine candidate...