A rapid and efficient in vitro propagation protocol of Acorus calamus L. an important endangered medicinal plant has been developed. Naturally grown rhizome segments were used as explants for initial culture. Surface sterilized rhizome segments were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations (0.5 mg l 3.0 mg l ) of cytokinins (BAP, Kn) and auxins (IAA, ...

Although, several pineapple micropropagation protocols have already been published, significant improvement could be achieved, if the stages of in vitro culture were better defined. Our work concerned several experiments aiming at the mass production of high quality plantlets. Tissue culture experiments were therefore conducted to develop rapid multiplication procedures for Ananas comosus L. (M...

Pyridine, a suspected genotoxic and formaldehyde, a potent carcinogen, are present in several industrial wastewater including commercial solvent, resin and pesticide manufacturing industries. The extent of pyridine and formaldehyde toxicity in aquatic organisms is unknown. Therefore, in present study the toxicity of pyridine and formaldehyde were investigated in artificial aquatic ecosystem (mi...

an efficient method for producing doubled haploid plants of date palm ( phoenix dactylifera l.) was established using in vitro colchicine treatment of anthers. to this study male inflorescences were harvested in the appropriate developmental stage. separated anthers after one day cold (4°c) pretreatment, in uninucleate stage of microspore were transferred to the modified y 3 medium containing d...

sugar type and growth regulators are essential fundamental components of tissue culture media. in this study, different concentrations of some carbohydrate and growth regulators during anther culture and producing somatic embryo of datura stramonium l. was investigated in ms medium. the ms medium supplemented with 0.5 mgl-1 kinetin and 2 mgl-1 2,4-d, and also the medium supplemented with 0.25 m...

The present study was carried out to study the effect of different dosages of β-glucan suspension on immune response and disease resistance in Anabas testudineus spawns against a fungal pathogen Saprolegnia parasitica. Eight day old spawns were exposed for 3 h in four different dosages of β-glucan suspension in phosphate buffered saline at the rate of 0, 5, 10, 15 mg l(-1). The cell suspension ...